"Detection and enumeration of L. monocytogenes have been carried out in 92 Ready-to-Eat (RTE) seafood products, manufactured in different countries and marketed in Italy. Fifty-one strains were characterised by serotyping, multiplex PCRs for the presence of virulence genes and PFGE. Of the samples tested, 8.6% were contaminated at a level of less than 100 CFU\/g in 98% of the samples. Isolates were serotyped as 1\/2a, 1\/2b, 1\/2c and 4e, and presented all the virulence genes considered. PFGE revealed a high similarity among the isolates, suggesting the existence of a widespread population of L. monocytogenes clones in different geographical areas"
Molecular characterization of L. monocytogenes isolated from ready-to-eat seafood in italy / Mureddu, A; Conter, M; Meloni, Domenico; Piras, Francesca; Ianieri, A; Mazzette, Rina. - In: ITALIAN JOURNAL OF FOOD SCIENCE. - ISSN 1120-1770. - 23:(2011), pp. 106-117.
Molecular characterization of L. monocytogenes isolated from ready-to-eat seafood in italy
MELONI, Domenico;PIRAS, Francesca;MAZZETTE, Rina
2011-01-01
Abstract
"Detection and enumeration of L. monocytogenes have been carried out in 92 Ready-to-Eat (RTE) seafood products, manufactured in different countries and marketed in Italy. Fifty-one strains were characterised by serotyping, multiplex PCRs for the presence of virulence genes and PFGE. Of the samples tested, 8.6% were contaminated at a level of less than 100 CFU\/g in 98% of the samples. Isolates were serotyped as 1\/2a, 1\/2b, 1\/2c and 4e, and presented all the virulence genes considered. PFGE revealed a high similarity among the isolates, suggesting the existence of a widespread population of L. monocytogenes clones in different geographical areas"I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
