"Contamination of dairy products with Staphylococcus aureus can be of animal or human origin. The host pathogen. relationship is an important factor determining genetic polymorphism of the strains and their potential. virulence. The aim of the present study was to carry out an extensive characterization of virulence factors and. to study the genetic variability of S. aureus strains isolated from raw ewe's milk cheese. A total of 100 S. aureus. strains isolated from cheese samples produced in 10 artisan cheese factories were analyzed for the presence. of enterotoxins (sea-see) and enterotoxins-like genes (seh, sek, sel, sem, seo, sep), leukocidins, exfoliatins, haemolysins,. toxic shock syndrome toxin 1 (TSST-1) and the accessory gene regulator alleles (agr). Strains were. also typed using pulsed-field gel electrophoresis (PFGE). AMOVA analysis carried out on PFGE and PCR data. showed that the major component explaining genetic distance between strains was the dairy of origin. Of the. total isolates 81% had a pathogenicity profile ascribable to “animal” biovar while 16% could be related to. “human” biovar. The biovar allowed to estimate the most likely origin of the contamination. Minimum inhibitory. concentrations (MICs) of nine antimicrobial agents and the presence of the corresponding genes coding. for antibiotic resistance was also investigated. 18 strains carrying blaZ gene showed resistance to ampicillin. and penicillin and 6 strains carrying tetM gene were resistant to tetracycline. The presence of mecA gene. and methicillin resistance, typical of strains of human origin, was never detected. The results obtained in. the present study confirm that S. aureus contamination in artisan cheese production is mainly of animal. origin."
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|Titolo:||Virulence factors and genetic variability of Staphylococcus aureus strains isolated from raw sheep's milk cheese|
|Data di pubblicazione:||2012|
|Appare nelle tipologie:||1.1 Articolo in rivista|