Fleeces, carcass surface, mucosal gut and faeces samples, collected from 95 slaughtered sheep and lambs from three abattoirs, were examined. The aim of this study was: 1) to evaluate the prevalence of the Verocytotoxic E. coli (VTEC); 2) to obtain virulence profile (stx1, stx2, hlyA and eae) by multiplex PCR; 3) to define the ovine-specific serogroup pathogenic power for the humans. An overall prevalence of 11.1% (adults 14%, lambs 7.8%) was found by direct PCR test. The VTEC occurrence was 18.9% in fleeces, 14.7% on carcasses and 10.5% in mucosal gut. According to the multiplex PCR the following results were obtained: 21% of the isolates belonged to VTEC pathogroup, within 92% were EHEC; 37.9% were identified as EPEC pathogroup. Forty one % of the strains were negative for all the genes. None of the isolates belonged to O157 and 0146 serogroups, while the 57% resulted O91.

Caratterizzazione di E.coli patogeni isolati in matrici ovine / Mazzette, Rina; Busia, G; Mureddu, A; Mazza, R; Consolati, Sg; Meloni, D.. - In: ITALIAN JOURNAL OF FOOD SAFETY. - ISSN 2239-7132. - 1:4(2012), pp. 71-75. [10.4081/ijfs.2012.4.71]

Caratterizzazione di E.coli patogeni isolati in matrici ovine

MAZZETTE, Rina;CONSOLATI, Simonetta Gianna;MELONI, Domenico
2012

Abstract

Fleeces, carcass surface, mucosal gut and faeces samples, collected from 95 slaughtered sheep and lambs from three abattoirs, were examined. The aim of this study was: 1) to evaluate the prevalence of the Verocytotoxic E. coli (VTEC); 2) to obtain virulence profile (stx1, stx2, hlyA and eae) by multiplex PCR; 3) to define the ovine-specific serogroup pathogenic power for the humans. An overall prevalence of 11.1% (adults 14%, lambs 7.8%) was found by direct PCR test. The VTEC occurrence was 18.9% in fleeces, 14.7% on carcasses and 10.5% in mucosal gut. According to the multiplex PCR the following results were obtained: 21% of the isolates belonged to VTEC pathogroup, within 92% were EHEC; 37.9% were identified as EPEC pathogroup. Forty one % of the strains were negative for all the genes. None of the isolates belonged to O157 and 0146 serogroups, while the 57% resulted O91.
Caratterizzazione di E.coli patogeni isolati in matrici ovine / Mazzette, Rina; Busia, G; Mureddu, A; Mazza, R; Consolati, Sg; Meloni, D.. - In: ITALIAN JOURNAL OF FOOD SAFETY. - ISSN 2239-7132. - 1:4(2012), pp. 71-75. [10.4081/ijfs.2012.4.71]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/146146
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