Lactic acid bacteria (LAB) were isolated during the production and the ripening of Sardinian sausage, a typical Italian dry fermented sausage. Samples were taken at diﬀerent stages, and 112 strains were isolated. The isolates were characterized using the micromethod proposed by Font de Valdez et al. [Font de Valdez, G., Savoy de Giori, G., Oliver, G., & De Ruiz Holgado, A. P. (1993). Development and optimization of an expensive microsystem for the biochemical characterization of lactobacilli. Microbiologie Aliments Nutrition, 11, 215–219]. Schillinger and Luckes [Schillinger, U., & Lucke, F. K. (1987). Identiﬁcation of lactobacilli from meat and meat products. Food Microbiology. (4), 199–208] scheme and the biochemical patterns given by Bergeys Manual of Systematic Bacteriology [Bergeys Manual of Systematic Bacteriology (1986). Baltimore: William and Wilkins] were used for preliminary identiﬁcation. A PCR-based method was then used to conﬁrm the results. LAB were the dominant ﬂora during ripening. They consisted mainly of homofermentative mesophilic rods. Lactobacillus sakei (43,3%), Lactobacillus plantarum (16,6%) and Lactobacillus curvatus (13,3%) were the main isolates. The results of the biochemical identiﬁcation methods agreed well with those of PCR-based identiﬁcation (91% agreement).
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|Titolo:||Evolution and identification of lactic acid bacteria isolated during the ripening of Sardinian sausages|
|Data di pubblicazione:||2005|
|Appare nelle tipologie:||1.1 Articolo in rivista|