In this article we present a study of the variability of the concentration of 5-hydroxymethyl-2-furaldehyde (HMF) in natural royal jelly (RJ) as a function of its storage temperature (−18, 4, and 25 °C) and time (up to 9 months after harvesting). For this work HMF is evaluated using an RP-HPLC method we previously assessed. While all RJ samples stored at 4 and −18 °C always showed levels of HMF under the limit of detection (0.13 mg kg−1), samples kept at 25 °C showed an exponential increase in the concentration of HMF as a function of the storage time. This behavior and a number of desirable features of the analytical method used (ease of use in routine laboratories, availability of a complete validation protocol specifically developed for RJ, based on consolidated chemical knowledge) allow us to hypothesize the use of HMF as a possible, reliable freshness marker for RJ.
A possible freshness marker for royal jelly: Formation of 5-hydroxymethyl-2-furaldehyde as a function of storage temperature and time / Ciulu, Marco; Floris, Ignazio; Nurchi, Vm; Panzanelli, Angelo; Pilo, Maria Itria; Spano, Nadia; Sanna, Gavino. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 1520-5118. - 63:16(2015), pp. 4190-4195. [10.1021/acs.jafc.5b00873]
A possible freshness marker for royal jelly: Formation of 5-hydroxymethyl-2-furaldehyde as a function of storage temperature and time
CIULU, Marco;FLORIS, Ignazio;PANZANELLI, Angelo;PILO, Maria Itria;SPANO, Nadia;SANNA, Gavino
2015-01-01
Abstract
In this article we present a study of the variability of the concentration of 5-hydroxymethyl-2-furaldehyde (HMF) in natural royal jelly (RJ) as a function of its storage temperature (−18, 4, and 25 °C) and time (up to 9 months after harvesting). For this work HMF is evaluated using an RP-HPLC method we previously assessed. While all RJ samples stored at 4 and −18 °C always showed levels of HMF under the limit of detection (0.13 mg kg−1), samples kept at 25 °C showed an exponential increase in the concentration of HMF as a function of the storage time. This behavior and a number of desirable features of the analytical method used (ease of use in routine laboratories, availability of a complete validation protocol specifically developed for RJ, based on consolidated chemical knowledge) allow us to hypothesize the use of HMF as a possible, reliable freshness marker for RJ.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
