Several mutations in genes involved in Saccharomyces mating type switching may affect the homothallic behaviour in wine yeasts. In this study the semi-homothallic (Hq) segregation of a flor wine yeast strain was analysed. We aimed to understand the molecular basis of this behaviour in a flor autochthonous strain, verifying the MAT locus status by a PCR-based HO gene disruption and sequencing of the Y region of the HML, HMR and MAT loci, after nested PCR. Presence of ORFs a1 and a2 in the Y region of the HML locus was found. At the ORF a2 at HML locus, a mutation in the stop codon was found, so the a2 ORF contains 33 more bases.
Diversity of Y region at HML locus in a Saccharomyces cerevisiae strain isolated from a Sardinian wine / Pirino, G; Zara, Severino; Pinna, G; Farris, Ga; Budroni, Marilena. - In: ANTONIE VAN LEEUWENHOEK. - ISSN 0003-6072. - 85:1(2004), pp. 29-36. [10.1023/B:ANTO.0000020269.80900.45]
Diversity of Y region at HML locus in a Saccharomyces cerevisiae strain isolated from a Sardinian wine
ZARA, Severino;BUDRONI, Marilena
2004-01-01
Abstract
Several mutations in genes involved in Saccharomyces mating type switching may affect the homothallic behaviour in wine yeasts. In this study the semi-homothallic (Hq) segregation of a flor wine yeast strain was analysed. We aimed to understand the molecular basis of this behaviour in a flor autochthonous strain, verifying the MAT locus status by a PCR-based HO gene disruption and sequencing of the Y region of the HML, HMR and MAT loci, after nested PCR. Presence of ORFs a1 and a2 in the Y region of the HML locus was found. At the ORF a2 at HML locus, a mutation in the stop codon was found, so the a2 ORF contains 33 more bases.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
