Flor yeasts ofSaccharomyces cerevisiaehave an innate diversity of FLO11 which codes for a highly hydrophobic and anionic cell-wall glycoprotein with a fundamental role in biofilm formation. In this study, 380 nitrogen compounds were administered to threeS. cerevisiaeflor strains handlingFLO11alleles with different expression levels.S. cerevisiaestrain S288c was used as the reference strain as it cannot produceFLO11p. The flor strains generally metabolized amino acids and dipeptides as the sole nitrogen source, although with some exceptions regarding L-histidine and histidine containing dipeptides. L-histidine completely inhibited growth and its effect on viability was inversely related toFLO11expression. Accordingly, L-histidine did not affect the viability of the Dflo11 and S288c strains. Also, L-histidine dramatically decreased air–liquid biofilm formation and adhesion to polystyrene of the flor yeasts with no effect on the transcription level of theFLO11gene. Moreover, L-histidine modified the chitin and glycans content on the cell-wall of flor yeasts. These findings reveal a novel biological activity of L-histidine in controlling the multicellular behavior of yeasts.
L-histidine inhibits biofilm formation and FLO11-associated phenotypes in Saccharomyces cerevisiae flor yeasts / Zara, Giacomo; Mannazzu, Ilaria Maria; Budroni, Marilena; Zara, Severino; Viti, Carlo; Decorosi, Francesca; Bou Zeidan, Marc; Giovannetti, Luciana. - In: PLOS ONE. - ISSN 1932-6203. - 9:11(2014). [10.1371/journal.pone.0112141]
L-histidine inhibits biofilm formation and FLO11-associated phenotypes in Saccharomyces cerevisiae flor yeasts
Zara, Giacomo;Mannazzu, Ilaria Maria;Budroni, Marilena;Zara, Severino;
2014-01-01
Abstract
Flor yeasts ofSaccharomyces cerevisiaehave an innate diversity of FLO11 which codes for a highly hydrophobic and anionic cell-wall glycoprotein with a fundamental role in biofilm formation. In this study, 380 nitrogen compounds were administered to threeS. cerevisiaeflor strains handlingFLO11alleles with different expression levels.S. cerevisiaestrain S288c was used as the reference strain as it cannot produceFLO11p. The flor strains generally metabolized amino acids and dipeptides as the sole nitrogen source, although with some exceptions regarding L-histidine and histidine containing dipeptides. L-histidine completely inhibited growth and its effect on viability was inversely related toFLO11expression. Accordingly, L-histidine did not affect the viability of the Dflo11 and S288c strains. Also, L-histidine dramatically decreased air–liquid biofilm formation and adhesion to polystyrene of the flor yeasts with no effect on the transcription level of theFLO11gene. Moreover, L-histidine modified the chitin and glycans content on the cell-wall of flor yeasts. These findings reveal a novel biological activity of L-histidine in controlling the multicellular behavior of yeasts.File | Dimensione | Formato | |
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