This paper introduces a new automatic system, of counting based on the elaboration of the digital images of cellular colonies grown on petri dishes. This system is mainly based on the region-growing algorithms for the recognition of the Regions Of Interest (ROI) in the image and Sanger's neural network for the characterization of such regions. Moreover a recognition of the most important filters is made in alternative respect; to region-growing approach. The new Graphics Users Interface is introduced. The better final classification is supplied from a Feed-Forward Neural Net (FF-NN) and compared with the K-Nearest Neighbour (K-NN). The results oil large dataset of ROIs are shown.
Automatic cell colony counting by region-growing approach / Masala, Gl; Bottigli, U; Brunetti, Antonio; Carpinelli, Massimo; Diaz, N; Fiori, Pier Luigi; Golosio, B; Oliva, Piernicola; Stegel, Giovanni. - In: IL NUOVO CIMENTO DELLA SOCIETÀ ITALIANA DI FISICA. C, GEOPHYSICS AND SPACE PHYSICS. - ISSN 1124-1896. - 30:6(2007), pp. 633-644. [10.1393/ncc/i2007-10273-3]