Highly purified 5-l-methyltetrahydrofolate (m-THF) and 5-l-formyl-THF (f- THF) preparations were compared for rescuing from methotrexate (MTX) toxicity in DBA2 mice transplanted with L1210 leukemia. Mice received two doses of reduced folates (2 mg/kg, s.c.) 16 and 24 h after a single s.c. MTX dose. f- THF was 1.8 time more effective than m-THF in protecting tumor cells from MTX (800 mg/kg). This MTX dose caused a 57% fall in circulating polymorphonucleates, which was prevented by both reduced folates. Treatment with 800 mg/kg of MTX plus m-THF was 1.5 fold more effective than the same MTX dose plus f-THF in increasing survival time of tumor-bearing mice. These data suggest a higher selectivity and efficacy of l-m-THF with respect to l- f-THF in rescuing from MTX toxicity.

1-5-formyltetrahydrofolate and 1-5-methyltetrahydrofolate rescue in L-1210 leukemia treated with high methotrexate doses / SIMILE MM; DE MIGLIO MR; NUFRIS A; PASCALE RM; MURONI MR; FEO F. - In: RESEARCH COMMUNICATIONS IN CHEMICAL PATHOLOGY AND PHARMACOLOGY. - ISSN 0034-5164. - 81:(1993), pp. 251-254.

1-5-formyltetrahydrofolate and 1-5-methyltetrahydrofolate rescue in L-1210 leukemia treated with high methotrexate doses

SIMILE, Maria Maddalena;DE MIGLIO, Maria Rosaria;PASCALE, Rosa Maria;MURONI, Maria Rosaria;
1993

Abstract

Highly purified 5-l-methyltetrahydrofolate (m-THF) and 5-l-formyl-THF (f- THF) preparations were compared for rescuing from methotrexate (MTX) toxicity in DBA2 mice transplanted with L1210 leukemia. Mice received two doses of reduced folates (2 mg/kg, s.c.) 16 and 24 h after a single s.c. MTX dose. f- THF was 1.8 time more effective than m-THF in protecting tumor cells from MTX (800 mg/kg). This MTX dose caused a 57% fall in circulating polymorphonucleates, which was prevented by both reduced folates. Treatment with 800 mg/kg of MTX plus m-THF was 1.5 fold more effective than the same MTX dose plus f-THF in increasing survival time of tumor-bearing mice. These data suggest a higher selectivity and efficacy of l-m-THF with respect to l- f-THF in rescuing from MTX toxicity.
1-5-formyltetrahydrofolate and 1-5-methyltetrahydrofolate rescue in L-1210 leukemia treated with high methotrexate doses / SIMILE MM; DE MIGLIO MR; NUFRIS A; PASCALE RM; MURONI MR; FEO F. - In: RESEARCH COMMUNICATIONS IN CHEMICAL PATHOLOGY AND PHARMACOLOGY. - ISSN 0034-5164. - 81:(1993), pp. 251-254.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/45542
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