Two components of the HERV-W family of human endogenous retroviruses are activated during multiple sclerosis (MS) and proposed immunopathogenic co-factors: MSRV (MS-associated retrovirus), and ERVWE1 (whose env protein, syncytin-1, reaches the plasma membrane). MSRVenv and syncytin-1 are closely related, and difficult to distinguish each other. The sequences of extracellular MSRVenv and of syncytin-1 available in GenBank were compared with those found in MS patients and controls of the cohort under study. With respect to syncytin-1, MSRVenv sequences have a 12-nucleotide insertion in the trans-membrane moiety. Based on this insertion, discriminatory real-time PCR assays were developed, that can amplify selectively either MSRVenv or syncytin-1. The data of MS patients and controls indicated that MSRV and ERVWE1 are both expressed in the brain of MS patients, while only MSRV is present in the blood; MSRV was released in culture by PBMCs of MSRV-producer individuals. These cells expressed the complete MSRVenv gene in the absence of syncytin-1 expression, up to the final, fully glycosylated envelope protein product, since western blot staining with anti-HERV-Wenv antibody detected two bands of the same molecular weight (73 and 61kDa) of the fully glycosylated and partially glycosylated HERV-Wenv uncleaved proteins. Beyond MSRVenv DNA copy numbers were more abundant in MS patients than in healthy humans, while syncytin-1 were unchanged. These findings reinforce the link between MSRV and MS.

Novel reliable real-time PCR for differential detection of MSRVenv and Syncytin-1 in RNA and DNA from patients with multiple sclerosis / Mameli, Giuseppe; Poddighe, L; Astone, V; Delogu, G; Arru, Giannina; Sotgiu, Stefano; Serra, Caterina; Dolei, Antonina. - In: JOURNAL OF VIROLOGICAL METHODS. - ISSN 0166-0934. - 161(1):98-106.:10(2009), pp. 98-106. [10.1016/j.jviromet.2009.05.024]

Novel reliable real-time PCR for differential detection of MSRVenv and Syncytin-1 in RNA and DNA from patients with multiple sclerosis

MAMELI, Giuseppe;ARRU, Giannina;SOTGIU, Stefano;SERRA, Caterina;DOLEI, Antonina
2009-01-01

Abstract

Two components of the HERV-W family of human endogenous retroviruses are activated during multiple sclerosis (MS) and proposed immunopathogenic co-factors: MSRV (MS-associated retrovirus), and ERVWE1 (whose env protein, syncytin-1, reaches the plasma membrane). MSRVenv and syncytin-1 are closely related, and difficult to distinguish each other. The sequences of extracellular MSRVenv and of syncytin-1 available in GenBank were compared with those found in MS patients and controls of the cohort under study. With respect to syncytin-1, MSRVenv sequences have a 12-nucleotide insertion in the trans-membrane moiety. Based on this insertion, discriminatory real-time PCR assays were developed, that can amplify selectively either MSRVenv or syncytin-1. The data of MS patients and controls indicated that MSRV and ERVWE1 are both expressed in the brain of MS patients, while only MSRV is present in the blood; MSRV was released in culture by PBMCs of MSRV-producer individuals. These cells expressed the complete MSRVenv gene in the absence of syncytin-1 expression, up to the final, fully glycosylated envelope protein product, since western blot staining with anti-HERV-Wenv antibody detected two bands of the same molecular weight (73 and 61kDa) of the fully glycosylated and partially glycosylated HERV-Wenv uncleaved proteins. Beyond MSRVenv DNA copy numbers were more abundant in MS patients than in healthy humans, while syncytin-1 were unchanged. These findings reinforce the link between MSRV and MS.
2009
Novel reliable real-time PCR for differential detection of MSRVenv and Syncytin-1 in RNA and DNA from patients with multiple sclerosis / Mameli, Giuseppe; Poddighe, L; Astone, V; Delogu, G; Arru, Giannina; Sotgiu, Stefano; Serra, Caterina; Dolei, Antonina. - In: JOURNAL OF VIROLOGICAL METHODS. - ISSN 0166-0934. - 161(1):98-106.:10(2009), pp. 98-106. [10.1016/j.jviromet.2009.05.024]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/45153
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