Cryopreservation of cells, tissues or organisms (i.e. the storage at liquid-nitrogen or nitrogen vapour temperature) offers the most secure form of preservation, allowing cells to be maintained unaltered for a long time and preventing them from ageing. On the other hand, the multi-year exposure of cryo-preserved cells (like: stem and germ cells) to the environmental γ-ray background might induce a radiation-damage accumulation, due to the inhibition of cellular repair mechanisms, and contribute to cancer or non-cancer pathology risk assessment when such cells should be transplanted in individuals. To investigate the effect of the cryo-preservation on the cell response to radiation exposures, the response of different biological systems (bacteria and mammalian cells) to γ-rays has been evaluated after irradiation in cryo-preserved and culture conditions, as a function of dose and doserate, in terms of a variety of cellular and molecular end-points.
Effect of cryo-preservation on the response of different biological systems to γ-ray exposure: a feasibility study / Bottigli, U; Brunetti, Antonio; Carpinelli, Massimo; Diaz, N; Fiori, Pier Luigi; Golosio, B; Masala, G. L; Oliva, Piernicola; Randaccio, P; Santona, S; Zamai, L; Centis, F; Valentini, M; Cesarini, W; Canonico, B; Grianti', F; Balata, M; Nisi, S; Laubenstein, M; Papa, S; Bedogni, R; Esposito, A; Cherubini, R; DE NADAL, V; Gerardi, S.. - In: IL NUOVO CIMENTO DELLA SOCIETÀ ITALIANA DI FISICA. C, GEOPHYSICS AND SPACE PHYSICS. - ISSN 1124-1896. - 31:1(2008), pp. 11-20. [10.1393/ncc/i2008-10276-6]
Effect of cryo-preservation on the response of different biological systems to γ-ray exposure: a feasibility study
BRUNETTI, Antonio;CARPINELLI, Massimo;FIORI, Pier Luigi;OLIVA, Piernicola;
2008-01-01
Abstract
Cryopreservation of cells, tissues or organisms (i.e. the storage at liquid-nitrogen or nitrogen vapour temperature) offers the most secure form of preservation, allowing cells to be maintained unaltered for a long time and preventing them from ageing. On the other hand, the multi-year exposure of cryo-preserved cells (like: stem and germ cells) to the environmental γ-ray background might induce a radiation-damage accumulation, due to the inhibition of cellular repair mechanisms, and contribute to cancer or non-cancer pathology risk assessment when such cells should be transplanted in individuals. To investigate the effect of the cryo-preservation on the cell response to radiation exposures, the response of different biological systems (bacteria and mammalian cells) to γ-rays has been evaluated after irradiation in cryo-preserved and culture conditions, as a function of dose and doserate, in terms of a variety of cellular and molecular end-points.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.