Background Experimental evidence indicates that Naloxone (NLX) holds antioxidant properties. The present study aims at verifying the hypothesis that NLX could prevent oxidative stress induced by hydrogen peroxide (H2O2) in PC12 cells. Methods To investigate the antioxidant effect of NLX, initially, we performed electrochemical experiments by means of platinum-based sensors in a cell-free system. Subsequently, NLX was tested in PC12 cells on H2O2-induced overproduction of intracellular levels of reactive-oxygen-species (ROS), apoptosis, modification of cells' cycle distribution and damage of cells’ plasma membrane. Results This study reveals that NLX counteracts intracellular ROS production, reduces H2O2-induced apoptosis levels, and prevents the oxidative damage-dependent increases of the percentage of cells in G2/M phase. Likewise, NLX protects PC12 cells from H2O2- induced oxidative damage, by preventing the lactate dehydrogenase (LDH) release. Moreover, electrochemical experiments confirmed the antioxidant properties of NLX. Conclusion Overall, these findings provide a starting point for studying further the protective effects of NLX on oxidative stress.

New perspective for an old drug: Can naloxone be considered an antioxidant agent? / Migheli, Rossana; Lostia, Giada; Galleri, Grazia; Rocchitta, Gaia Giovanna Maria; Serra, Pier Andrea; Campesi, Ilaria; Bassareoc, Valentina; Acquas, Elio; Peana, Alessandra T.. - In: BIOCHEMISTRY AND BIOPHYSICS REPORTS. - ISSN 2405-5808. - 34:(2023), p. 101441. [10.1016/j.bbrep.2023.101441]

New perspective for an old drug: Can naloxone be considered an antioxidant agent?

Rossana Migheli
;
Giada Lostia;Grazia Galleri;Gaia Rocchitta;Pier Andrea Serra;Ilaria Campesi;Alessandra T. Peana
2023-01-01

Abstract

Background Experimental evidence indicates that Naloxone (NLX) holds antioxidant properties. The present study aims at verifying the hypothesis that NLX could prevent oxidative stress induced by hydrogen peroxide (H2O2) in PC12 cells. Methods To investigate the antioxidant effect of NLX, initially, we performed electrochemical experiments by means of platinum-based sensors in a cell-free system. Subsequently, NLX was tested in PC12 cells on H2O2-induced overproduction of intracellular levels of reactive-oxygen-species (ROS), apoptosis, modification of cells' cycle distribution and damage of cells’ plasma membrane. Results This study reveals that NLX counteracts intracellular ROS production, reduces H2O2-induced apoptosis levels, and prevents the oxidative damage-dependent increases of the percentage of cells in G2/M phase. Likewise, NLX protects PC12 cells from H2O2- induced oxidative damage, by preventing the lactate dehydrogenase (LDH) release. Moreover, electrochemical experiments confirmed the antioxidant properties of NLX. Conclusion Overall, these findings provide a starting point for studying further the protective effects of NLX on oxidative stress.
2023
New perspective for an old drug: Can naloxone be considered an antioxidant agent? / Migheli, Rossana; Lostia, Giada; Galleri, Grazia; Rocchitta, Gaia Giovanna Maria; Serra, Pier Andrea; Campesi, Ilaria; Bassareoc, Valentina; Acquas, Elio; Peana, Alessandra T.. - In: BIOCHEMISTRY AND BIOPHYSICS REPORTS. - ISSN 2405-5808. - 34:(2023), p. 101441. [10.1016/j.bbrep.2023.101441]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/303006
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