Analyte quantification in first generation electrochemical biosensors is threatened by electro-active molecules, such as ascorbic acid (AA). Electrochemical deposition of ortho-phenylendiamine (oPD) on transducer considerably reduces AA interfering. These properties of poly-oPD (PPD) are influenced by electro-polymerization conditions. Chronoamperometry (CA) is proposed as an alternative technique for oPD electro-deposition over Pt. Pt/PPD sensors are evaluated through functional parameters related to AA rejection and analytical performances. N2, O2, and air bubbled supporting electrolyte and several step duration times are used. Best performing CA-PPD sensor is obtained in N2-purged phosphate solution containing 300 mM oPD by means of a 1 s step duration/120 steps CA. High performing PPD is achieved in shorter time compared to commonly used constant potential amperometry. Aging of polymeric features and scanning electron microscopy investigations are performed and optimized CA electro-polymerization conditions are used to build up an efficient interference blocking layer in a glucose oxidase biosensor

Chronoamperometry as effective alternative technique for electro-synthesis of ortho-phenylendiamine permselective films for biosensor applications / Monti, Patrizia; Bacciu, Andrea; Arrigo, Paola; Marceddu, Salvatore; Migheli, Quirico; Serra, Pier Andrea; Rocchitta, Gaia Giovanna Maria. - In: JOURNAL OF APPLIED POLYMER SCIENCE. - ISSN 0021-8995. - 137:39(2020), p. 49172. [10.1002/app.49172]

Chronoamperometry as effective alternative technique for electro-synthesis of ortho-phenylendiamine permselective films for biosensor applications

Patrizia Monti
;
Andrea Bacciu;Paola Arrigo;Quirico Migheli;Pier Andrea Serra
;
Gaia Rocchitta
2020

Abstract

Analyte quantification in first generation electrochemical biosensors is threatened by electro-active molecules, such as ascorbic acid (AA). Electrochemical deposition of ortho-phenylendiamine (oPD) on transducer considerably reduces AA interfering. These properties of poly-oPD (PPD) are influenced by electro-polymerization conditions. Chronoamperometry (CA) is proposed as an alternative technique for oPD electro-deposition over Pt. Pt/PPD sensors are evaluated through functional parameters related to AA rejection and analytical performances. N2, O2, and air bubbled supporting electrolyte and several step duration times are used. Best performing CA-PPD sensor is obtained in N2-purged phosphate solution containing 300 mM oPD by means of a 1 s step duration/120 steps CA. High performing PPD is achieved in shorter time compared to commonly used constant potential amperometry. Aging of polymeric features and scanning electron microscopy investigations are performed and optimized CA electro-polymerization conditions are used to build up an efficient interference blocking layer in a glucose oxidase biosensor
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/232010
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