Highly sensitive, with high temporal resolution, detection of ethanol concentrations in discrete brain regions of rats voluntarily accessing ethanol would represent a source of greatly desirable data in studies devoted to understanding the kinetics of the neurobiological basis of ethanol’s ability to impact on behaviour. In the present study we present a series of experiments aiming to validate and apply an original high-tech implantable device, consisting of the coupling, for the first time, of an amperometric biosensor for brain ethanol detection, with a sensor for detecting the micro vibrations of the animal. This device allows the real-time comparison between the ethanol intake, its cerebral concentrations, and their effect on the motion when the animal is in the condition of voluntary drinking. To this end, we assessed in-vitro the efficiency of three different biosensor designs loading diverse alcohol oxidase enzymes (AOx) obtained from three different AOx-donor strains: Hansenula polymorpha, Candida boidinii and Pichia pastoris. In-vitro data disclosed that the devices loading Hansenula polymorpha and Candida boidinii were similarly efficient (respectively, LRS: 1.98±0.07 and 1.38±0.04 nA mM−1) but significantly less than the Pichia pastoris-loaded one (LRS: 7.57±0.12 nA mM−1). The in-vivo results indicate that this last biosensor design detected the rise of ethanol in the nucleus accumbens shell (AcbSh) after 15 minutes of voluntary 10% ethanol solution intake. At the same time, the micro-vibration sensor detected a significant increase in the rat’s motion signal. Notably, both the biosensor and micro-vibration sensor described similar and parallel time-dependent U-shaped curves, thus providing a highly sensitive and time-locked high-resolution detection of the neurochemical and behavioural kinetics upon voluntary ethanol intake. The results overall indicate that such a dual telemetry unit represents a powerful device which, implanted in different brain areas, may boost further investigations on the neurobiological mechanisms that underlie ethanol-induced motor activity and reward.

Simultaneous wireless and high-resolution detection of nucleus accumbens shell ethanol concentrations and free motion of rats upon voluntary ethanol intake / Rocchitta, Gaia Giovanna Maria; Peana, Alessandra Tiziana; Bazzu, Gianfranco; Cossu, Alberto; Carta, Sara; Arrigo, Paola; Bacciu, Andrea; Migheli, Rossana; Farina, Donatella; Zinellu, Manuel; Acquas, Elio; Serra, Pier Andrea. - In: ALCOHOL. - ISSN 0741-8329. - 78:(2019), pp. 69-78. [10.1016/j.alcohol.2019.04.002]

Simultaneous wireless and high-resolution detection of nucleus accumbens shell ethanol concentrations and free motion of rats upon voluntary ethanol intake

Rocchitta, G.
Validation
;
Peana, A. T.
Writing – Original Draft Preparation
;
Bazzu, G.
Methodology
;
Arrigo, P.
Membro del Collaboration Group
;
Bacciu, A.
Membro del Collaboration Group
;
Migheli, R.
Membro del Collaboration Group
;
Farina, D.
Membro del Collaboration Group
;
Zinellu, M.
Membro del Collaboration Group
;
Serra, P. A.
Writing – Review & Editing
2019

Abstract

Highly sensitive, with high temporal resolution, detection of ethanol concentrations in discrete brain regions of rats voluntarily accessing ethanol would represent a source of greatly desirable data in studies devoted to understanding the kinetics of the neurobiological basis of ethanol’s ability to impact on behaviour. In the present study we present a series of experiments aiming to validate and apply an original high-tech implantable device, consisting of the coupling, for the first time, of an amperometric biosensor for brain ethanol detection, with a sensor for detecting the micro vibrations of the animal. This device allows the real-time comparison between the ethanol intake, its cerebral concentrations, and their effect on the motion when the animal is in the condition of voluntary drinking. To this end, we assessed in-vitro the efficiency of three different biosensor designs loading diverse alcohol oxidase enzymes (AOx) obtained from three different AOx-donor strains: Hansenula polymorpha, Candida boidinii and Pichia pastoris. In-vitro data disclosed that the devices loading Hansenula polymorpha and Candida boidinii were similarly efficient (respectively, LRS: 1.98±0.07 and 1.38±0.04 nA mM−1) but significantly less than the Pichia pastoris-loaded one (LRS: 7.57±0.12 nA mM−1). The in-vivo results indicate that this last biosensor design detected the rise of ethanol in the nucleus accumbens shell (AcbSh) after 15 minutes of voluntary 10% ethanol solution intake. At the same time, the micro-vibration sensor detected a significant increase in the rat’s motion signal. Notably, both the biosensor and micro-vibration sensor described similar and parallel time-dependent U-shaped curves, thus providing a highly sensitive and time-locked high-resolution detection of the neurochemical and behavioural kinetics upon voluntary ethanol intake. The results overall indicate that such a dual telemetry unit represents a powerful device which, implanted in different brain areas, may boost further investigations on the neurobiological mechanisms that underlie ethanol-induced motor activity and reward.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/220783
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