L-glutamate is one the most important excitatory neurotransmitter at central nervous system level and it is implicated in several pathologies. So, it is very important to monitor its variations, in real time in animal models’ brain. The present study aimed to develop and characterize a new amperometric glutamate biosensor design that exploits the selectivity of Glutamate Oxidase (GluOx) for l-glutamate, and the capability of a small molecule as propylene glycol (PG), never used before, to influence and extend the stability and the activity of enzyme. Different designs were evaluated by modifying the main components in their concentrations to find the most suitable design. Moreover, enzyme concentrations from 100 U/ml up to 200 U/ml were verified and different PG concentrations (1%, 0.1% and 0.05%) were tested. The most suitable selected design was Ptc/PPD/PEI(1%)2/GlutOx5/PG(0.1%) and it was compared to the same already described design loading PEDGE, instead of PG, in terms of over-time performances. PG has proved to be capable of determining an over-time stability of the glutamate biosensor in particular in terms of linear region slope (LRS) up to 21 days.

Propylene glycol stabilizes the linear response of glutamate biosensor: potential implications for in-vivo neurochemical monitoring / Rocchitta, Gaia Giovanna Maria; Bacciu, Andrea; Arrigo, Paola; Migheli, Rossana; Bazzu, Gianfranco; Serra, Pier Andrea. - In: CHEMOSENSORS. - ISSN 2227-9040. - 6:4(2018), p. 58. [10.3390/chemosensors6040058]

Propylene glycol stabilizes the linear response of glutamate biosensor: potential implications for in-vivo neurochemical monitoring

Gaia Rocchitta
Writing – Original Draft Preparation
;
BACCIU, ANDREA
Validation
;
Paola Arrigo
Validation
;
Rossana Migheli
Formal Analysis
;
Gianfranco Bazzu
Formal Analysis
;
Pier Andrea Serra
Writing – Review & Editing
2018

Abstract

L-glutamate is one the most important excitatory neurotransmitter at central nervous system level and it is implicated in several pathologies. So, it is very important to monitor its variations, in real time in animal models’ brain. The present study aimed to develop and characterize a new amperometric glutamate biosensor design that exploits the selectivity of Glutamate Oxidase (GluOx) for l-glutamate, and the capability of a small molecule as propylene glycol (PG), never used before, to influence and extend the stability and the activity of enzyme. Different designs were evaluated by modifying the main components in their concentrations to find the most suitable design. Moreover, enzyme concentrations from 100 U/ml up to 200 U/ml were verified and different PG concentrations (1%, 0.1% and 0.05%) were tested. The most suitable selected design was Ptc/PPD/PEI(1%)2/GlutOx5/PG(0.1%) and it was compared to the same already described design loading PEDGE, instead of PG, in terms of over-time performances. PG has proved to be capable of determining an over-time stability of the glutamate biosensor in particular in terms of linear region slope (LRS) up to 21 days.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/218287
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