A method for ‘in vivo’ determination of the oxytetracyclin residues in ovine milk at low levels is described. Two groups of Sardinian breed sheep were treated with a dose of oxytetracycline by intramammary infusion and intramuscular administration, respectively. Oxytetracycline residues in extracts obtained from a preliminary cleanup procedure, were detected by an isocratic high-performance liquid chromatography (HPLC) method. Linear calibration plots were obtained over a large concentration range of 1 mg ml1–10 ng ml1, with correlation coefficients higher than 0.996. Recoveries between 85.8 and 98.9% were obtained. Limit of detection (LOD) and limit of determination (LOQ) were 5.2 and 17.5 ng ml1, respectively. This method would be useful for routine monitoring of oxytetracycline residues in ovine dairy milk.
Monitoring of oxytetracycline in ovine milk by HPLC / Boatto, Gianpiero; Pau, Amedeo; Palomba, M.; Arenare, L.; Cerri, R.. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 20:(1999), pp. 321-326. [10.1016/S0731-7085(99)00050-3]
Monitoring of oxytetracycline in ovine milk by HPLC
BOATTO, Gianpiero;PAU, Amedeo;PALOMBA M.;
1999-01-01
Abstract
A method for ‘in vivo’ determination of the oxytetracyclin residues in ovine milk at low levels is described. Two groups of Sardinian breed sheep were treated with a dose of oxytetracycline by intramammary infusion and intramuscular administration, respectively. Oxytetracycline residues in extracts obtained from a preliminary cleanup procedure, were detected by an isocratic high-performance liquid chromatography (HPLC) method. Linear calibration plots were obtained over a large concentration range of 1 mg ml1–10 ng ml1, with correlation coefficients higher than 0.996. Recoveries between 85.8 and 98.9% were obtained. Limit of detection (LOD) and limit of determination (LOQ) were 5.2 and 17.5 ng ml1, respectively. This method would be useful for routine monitoring of oxytetracycline residues in ovine dairy milk.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
