The yeast Hansenula polymorpha is able to grow on vanadate concentrations that are toxic to other organisms. Transmission electron microscopy analysis showed that H. polymorpha cells growing on a vanadate-containing medium undergo a significant increase in cell vacuolation and a thickening of the cell wall; the presence of small cytoplasmic vesicles and an increase in cristae at the level of the plasma membrane were also observed. These ultrastructural modifications were accompanied by a change in the intracellular polyphosphate level, as shown by in vivo 31P-NMR. The involvement of these observed changes in vanadium detoxification is discussed.
Vanadium affects vacuolation and phosphate metabolism in Hansenula polymorpha / Mannazzu, Ilaria Maria; Guerra, E; Strabbioli, R; Masia, A; Maestrale, Gb; Zoroddu, Maria Antonietta; Fatichenti, Fabrizio. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 147:(1997), pp. 23-28.
Vanadium affects vacuolation and phosphate metabolism in Hansenula polymorpha
MANNAZZU, Ilaria Maria
;ZORODDU, Maria Antonietta;
1997-01-01
Abstract
The yeast Hansenula polymorpha is able to grow on vanadate concentrations that are toxic to other organisms. Transmission electron microscopy analysis showed that H. polymorpha cells growing on a vanadate-containing medium undergo a significant increase in cell vacuolation and a thickening of the cell wall; the presence of small cytoplasmic vesicles and an increase in cristae at the level of the plasma membrane were also observed. These ultrastructural modifications were accompanied by a change in the intracellular polyphosphate level, as shown by in vivo 31P-NMR. The involvement of these observed changes in vanadium detoxification is discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
