Opioid-binding sites were identified in highly purified nuclei isolated from hamster ventricular myocardial cells. A significant increase in the maximal binding capacity for a kappa opioid receptor ligand was observed in myocardial nuclei from BIO 14.6 cardiomyopathic hamsters, as compared with nuclei obtained from normal myocytes of the F1B strain. The exposure of isolated nuclei to dynorphin B, a natural agonist of kappa opioid receptors, markedly increased opioid peptide gene transcription. The transcriptional effect was mediated by nuclear protein kinase C activation and occurred at a higher rate in nuclei from cardiomyopathic myocytes than in nuclei isolated from normal cells. Thus, a nuclear endorphinergic system may play an intracrine role in the regulation of gene transcription under both normal and pathological conditions.

Nuclear opioid receptors activate opioid peptide gene transcription in isolated myocardial nuclei / Ventura, C; Maioli, Margherita; Pintus, Gianfranco; Posadino, Am; Tadolini, B.. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 273:22(1998), pp. 13383-13386. [10.1074/jbc.273.22.13383]

Nuclear opioid receptors activate opioid peptide gene transcription in isolated myocardial nuclei

MAIOLI, Margherita;PINTUS, Gianfranco;POSADINO AM;
1998

Abstract

Opioid-binding sites were identified in highly purified nuclei isolated from hamster ventricular myocardial cells. A significant increase in the maximal binding capacity for a kappa opioid receptor ligand was observed in myocardial nuclei from BIO 14.6 cardiomyopathic hamsters, as compared with nuclei obtained from normal myocytes of the F1B strain. The exposure of isolated nuclei to dynorphin B, a natural agonist of kappa opioid receptors, markedly increased opioid peptide gene transcription. The transcriptional effect was mediated by nuclear protein kinase C activation and occurred at a higher rate in nuclei from cardiomyopathic myocytes than in nuclei isolated from normal cells. Thus, a nuclear endorphinergic system may play an intracrine role in the regulation of gene transcription under both normal and pathological conditions.
Nuclear opioid receptors activate opioid peptide gene transcription in isolated myocardial nuclei / Ventura, C; Maioli, Margherita; Pintus, Gianfranco; Posadino, Am; Tadolini, B.. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 273:22(1998), pp. 13383-13386. [10.1074/jbc.273.22.13383]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/81268
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