The reactions of a few representative gold(III) complexes – [Au(ethylenediamine)2]Cl3, [Au(diethylentriamine)Cl]Cl2, [Au(1,4,8,11-tetraazacyclotetradecane)](ClO4)2Cl, [Au(2,2¢, 2¢-terpyridine)Cl]Cl2, [Au(2,2¢-bipyridine)(OH)2][PF6] and the organometallic compound [Au(6-(1,1-dimethylbenzyl)- 2,2¢-bipyridine-H)(OH)][PF6] – with BSA were investigated by the joint use of various spectroscopic methods and separation techniques. Weak metal–protein interactions were revealed for the [Au(ethylenediamine)2]3+ and [Au(1,4,8,11-tetraazacyclotetradecane)]3+ species, whereas progressive reduction of the gold(III) centre was observed in the cases of [Au(2,2¢-bipyridine)(OH)2]+ and [Au(2,2¢,2¢- terpyridine)Cl]2+. In contrast, tight metal–protein adducts are formed when BSA is reacted with either [Au(diethylentriamine) Cl]2+ and [Au(6-(1,1-dimethylbenzyl)-2,2¢-bipyridine- H)(OH)]+. Notably, binding of the latter complex to serum albumin results in the appearance of characteristic CDbands in the visible spectrum. It is suggested that adduct formation for both of these gold(III) complexes occurs through coordination at the level of surface histidines. Stability of these gold(III) complexes/serum albumin adducts was tested under physiologically relevant conditions and found to be appreciable.Metal binding to the protein is tight; complete detachment of themetal from the protein has been achieved only after the addition of excess potassium cyanide. The implications of the present results for the pharmacological activity of these novel cytotoxic agents are discussed.

Reactions of gold(III) complexes with serum albumin / Marcon, G; Messori, L; Orioli, P; Cinellu, Maria Agostina; Minghetti, G.. - In: EUROPEAN JOURNAL OF BIOCHEMISTRY. - ISSN 0014-2956. - 270:(2003), pp. 4655-4661.

Reactions of gold(III) complexes with serum albumin

CINELLU, Maria Agostina;
2003-01-01

Abstract

The reactions of a few representative gold(III) complexes – [Au(ethylenediamine)2]Cl3, [Au(diethylentriamine)Cl]Cl2, [Au(1,4,8,11-tetraazacyclotetradecane)](ClO4)2Cl, [Au(2,2¢, 2¢-terpyridine)Cl]Cl2, [Au(2,2¢-bipyridine)(OH)2][PF6] and the organometallic compound [Au(6-(1,1-dimethylbenzyl)- 2,2¢-bipyridine-H)(OH)][PF6] – with BSA were investigated by the joint use of various spectroscopic methods and separation techniques. Weak metal–protein interactions were revealed for the [Au(ethylenediamine)2]3+ and [Au(1,4,8,11-tetraazacyclotetradecane)]3+ species, whereas progressive reduction of the gold(III) centre was observed in the cases of [Au(2,2¢-bipyridine)(OH)2]+ and [Au(2,2¢,2¢- terpyridine)Cl]2+. In contrast, tight metal–protein adducts are formed when BSA is reacted with either [Au(diethylentriamine) Cl]2+ and [Au(6-(1,1-dimethylbenzyl)-2,2¢-bipyridine- H)(OH)]+. Notably, binding of the latter complex to serum albumin results in the appearance of characteristic CDbands in the visible spectrum. It is suggested that adduct formation for both of these gold(III) complexes occurs through coordination at the level of surface histidines. Stability of these gold(III) complexes/serum albumin adducts was tested under physiologically relevant conditions and found to be appreciable.Metal binding to the protein is tight; complete detachment of themetal from the protein has been achieved only after the addition of excess potassium cyanide. The implications of the present results for the pharmacological activity of these novel cytotoxic agents are discussed.
2003
Reactions of gold(III) complexes with serum albumin / Marcon, G; Messori, L; Orioli, P; Cinellu, Maria Agostina; Minghetti, G.. - In: EUROPEAN JOURNAL OF BIOCHEMISTRY. - ISSN 0014-2956. - 270:(2003), pp. 4655-4661.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/78480
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