The effects of organic system on Stearoyl-CoA desaturase (SCD) gene expression in milk somatic cells was evaluated in 30 pregnant pluriparous goats, divided into two homogeneous groups (O and S). Group O was fed according EC Regulation 834/2007 and led to pasture while group S was stabled and received alfalfa hay as forage. After kids weaning, milk yield was recorded and individual representative milk samples were analysed for chemical composition and fatty acid profile. The SCD gene expression was studied by extraction of mRNA from milk somatic cells and RT-PCR analysis. The body weight of goats of both groups did not show any significant change during the trial. Average milk yield did not differ statistically between the groups while milk from group O showed a significantly higher fat content than those from group S (65.9 vs. 54.3 g/day, P<0.01). Organic system significantly affected the percentages of c9 C18:1, t11 C18:1, octadecadienoic acid, octadecatrienoic acid, monounsaturated fatty acids as well as the c9t11 conjugated linoleic acid (CLA) (0.810 vs. 0.542 g/100g of fat, for group O and S, respectively, P<0.01) and ΣCLA (0.860 vs. 0.580 g/100g of fat for group O and S, respectively, P<0.01) concentrations in milk. The Stearoyl-CoA desaturase ratios were not different between treatments. Finally, the SCD gene expression was significantly higher in the somatic cells in milk yielded from the “organic” group (AU:1.5 ± 0.5 vs 0.39 ± 0.11 for group O and S, respectively, P<0.01).
Influence of organic systems on Stearoyl-CoA desaturase gene expression in goat milk / Tudisco, R.; Calabrò, S.; Cutrignelli, M. I.; Moniello, Giuseppe; Grossi, M.; Gonzalez, O. J.; Piccolo, V.; Infascelli, F.. - In: SMALL RUMINANT RESEARCH. - ISSN 0921-4488. - (2012), pp. 37-42. [10.1016/j.smallrumres.2012.04.031]
Influence of organic systems on Stearoyl-CoA desaturase gene expression in goat milk
MONIELLO, Giuseppe;
2012-01-01
Abstract
The effects of organic system on Stearoyl-CoA desaturase (SCD) gene expression in milk somatic cells was evaluated in 30 pregnant pluriparous goats, divided into two homogeneous groups (O and S). Group O was fed according EC Regulation 834/2007 and led to pasture while group S was stabled and received alfalfa hay as forage. After kids weaning, milk yield was recorded and individual representative milk samples were analysed for chemical composition and fatty acid profile. The SCD gene expression was studied by extraction of mRNA from milk somatic cells and RT-PCR analysis. The body weight of goats of both groups did not show any significant change during the trial. Average milk yield did not differ statistically between the groups while milk from group O showed a significantly higher fat content than those from group S (65.9 vs. 54.3 g/day, P<0.01). Organic system significantly affected the percentages of c9 C18:1, t11 C18:1, octadecadienoic acid, octadecatrienoic acid, monounsaturated fatty acids as well as the c9t11 conjugated linoleic acid (CLA) (0.810 vs. 0.542 g/100g of fat, for group O and S, respectively, P<0.01) and ΣCLA (0.860 vs. 0.580 g/100g of fat for group O and S, respectively, P<0.01) concentrations in milk. The Stearoyl-CoA desaturase ratios were not different between treatments. Finally, the SCD gene expression was significantly higher in the somatic cells in milk yielded from the “organic” group (AU:1.5 ± 0.5 vs 0.39 ± 0.11 for group O and S, respectively, P<0.01).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
