Infections due to enterohaemorrhagic E. coli (Escherichia coli) have a low incidence but can have severe and sometimes fatal health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl) triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 102 CFU/mL, constituting a simple and selective method for the effective screening of water samples.

FTIR nanobiosensors for Escherichia coli detection / Mura, Stefania; Greppi, Gianfranco; Marongiu, Maria Laura; Roggero, Pier Paolo; Ravindranath, Sp; Mauer, Lj; Schibeci, N; Perria, F; Massimo, Piccinini7; Innocenzi, Plinio; Joseph Irudayaraj, J.. - In: BEILSTEIN JOURNAL OF NANOTECHNOLOGY. - ISSN 2190-4286. - 3:(2012), pp. 485-492. [10.3762/bjnano.3.55]

FTIR nanobiosensors for Escherichia coli detection

MURA, Stefania;GREPPI, Gianfranco;MARONGIU, Maria Laura;ROGGERO, Pier Paolo;INNOCENZI, Plinio;
2012-01-01

Abstract

Infections due to enterohaemorrhagic E. coli (Escherichia coli) have a low incidence but can have severe and sometimes fatal health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl) triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 102 CFU/mL, constituting a simple and selective method for the effective screening of water samples.
2012
FTIR nanobiosensors for Escherichia coli detection / Mura, Stefania; Greppi, Gianfranco; Marongiu, Maria Laura; Roggero, Pier Paolo; Ravindranath, Sp; Mauer, Lj; Schibeci, N; Perria, F; Massimo, Piccinini7; Innocenzi, Plinio; Joseph Irudayaraj, J.. - In: BEILSTEIN JOURNAL OF NANOTECHNOLOGY. - ISSN 2190-4286. - 3:(2012), pp. 485-492. [10.3762/bjnano.3.55]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/62306
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 31
social impact