Twelve prepubertal (35-45-day-old) and 13 adult (3-6-year-old),Oats were stimulated and COCS were collected by OPU and matured in vitro. After oocyte in vitro fertilization and culture, blastocysts were vitrified/warmed and cultured in vitro to assess blastocoelic re-expansion and gone expression. Prepubertals produced more COCs (p < 0.01) than adults. Degenerated COCs number (p < 0.01) was higher in prepubertals than adults and lower cleavage (p < 0.01) and blastocyst rates (p < 0.05) were evidenced in prepubertal compared to adult fertilized oocytes. Prepubertal vitrified/warmed blastocysts present a lower viability (p < 0.05) than adults. No significant differences were found in the expression of Cyclin B1, E-caderin, Na/K-ATPase, HSP90 and Aquaporin 3 genes. In conclusion our results evidenced a low development and cryotolerance of prepubertal goat embryos. However, the quality of viable prepubertal ernbryos after vitrification do not differ from the adult counterpart.
Twelve prepubertal (35-45-day-old) and 13 adult (3-6-year-old),Oats were stimulated and COCS were collected by OPU and matured in vitro. After oocyte in vitro fertilization and culture, blastocysts were vitrified/warmed and cultured in vitro to assess blastocoelic re-expansion and gone expression. Prepubertals produced more COCs (p < 0.01) than adults. Degenerated COCs number (p < 0.01) was higher in prepubertals than adults and lower cleavage (p < 0.01) and blastocyst rates (p < 0.05) were evidenced in prepubertal compared to adult fertilized oocytes. Prepubertal vitrified/warmed blastocysts present a lower viability (p < 0.05) than adults. No significant differences were found in the expression of Cyclin B1, E-caderin, Na/K-ATPase, HSP90 and Aquaporin 3 genes. In conclusion our results evidenced a low development and cryotolerance of prepubertal goat embryos. However, the quality of viable prepubertal ernbryos after vitrification do not differ from the adult counterpart.
CRYOTOLERANCE AND GENE EXPRESSION OF IN VITRO PRODUCED PREPUBERTAL GOAT BLASTOCYSTS / Leoni, Giovanni Giuseppe; Succu, Sara; Satta, V; Bogliolo, Luisa; Bebbere, D; Spezzigu, A; Madeddu, M; Tedde, A; Berlinguer, Fiammetta; Ledda, Sergio; Naitana, S.. - In: COMPTES RENDUS DE L'ACADÉMIE BULGARE DES SCIENCES. - ISSN 1310-1331. - 62:3(2009), pp. 367-372.
CRYOTOLERANCE AND GENE EXPRESSION OF IN VITRO PRODUCED PREPUBERTAL GOAT BLASTOCYSTS
LEONI, Giovanni Giuseppe;SUCCU, Sara;BOGLIOLO, Luisa;Bebbere D;BERLINGUER, Fiammetta;LEDDA, Sergio;
2009-01-01
Abstract
Twelve prepubertal (35-45-day-old) and 13 adult (3-6-year-old),Oats were stimulated and COCS were collected by OPU and matured in vitro. After oocyte in vitro fertilization and culture, blastocysts were vitrified/warmed and cultured in vitro to assess blastocoelic re-expansion and gone expression. Prepubertals produced more COCs (p < 0.01) than adults. Degenerated COCs number (p < 0.01) was higher in prepubertals than adults and lower cleavage (p < 0.01) and blastocyst rates (p < 0.05) were evidenced in prepubertal compared to adult fertilized oocytes. Prepubertal vitrified/warmed blastocysts present a lower viability (p < 0.05) than adults. No significant differences were found in the expression of Cyclin B1, E-caderin, Na/K-ATPase, HSP90 and Aquaporin 3 genes. In conclusion our results evidenced a low development and cryotolerance of prepubertal goat embryos. However, the quality of viable prepubertal ernbryos after vitrification do not differ from the adult counterpart.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
