The present study was conducted to determine the effect of okadic acid (OA), a potent inhibitor of seronine/treonine 1 and 2A phosphatase, on meiotic resumption and progression in canine oocytes with different diameters. Cumulus-oocyte complexes were collected from ovaries of bitches at different oestrous phases. In Experiment 1, to determine the optimal concentration of OA (0.5 or 2 mu M), the oocytes were pre-incubated for 1, 3, and 20 h in TCM 199 supplemented with 20% SCE and thereafter cultured in the same medium without OA. In Experiment 2, the selected oocytes were divided into three groups according to their diameter: <110 mu m, 110-120 mu m, >120 mu m, and pre-incubated in OA 0.5 mu M for 1 h. Oocytes were cultured in vitro as previously described. After 72 h of IVM, in Experiment 1, significantly more oocytes reached MII stage with 0.5 mu M for 1 h (30.8% P<0.001%) for oocytes cultured in other OA condition and in control group. In Experiment 2, OA induced a significantly higher incidence of MII oocytes in the 110-120 mu m and >120 mu m groups (P<0.001) compared to control group, but a significantly higher proportion of the oocytes >120 mu m pre-incubated with OA progressed to MII (51.3% P<0.001). In contrast, smaller oocytes (<110) did not develop to MII stage with or without OA. In conclusion, treatment of canine oocytes with 0.5 mu M for 1 h, improves meiotic maturation. The culture of fully grown (>120 mu m) oocytes with OA at the onset of in vitro maturation can result in a higher frequency of meiotic maturation. (C) 2012 Elsevier Inc. All rights reserved.

The effect of okadaic acid on meiotic maturation of canine oocytes of different size / Ariu, F; Fois, S; Bebbere, Daniela; Ledda, Sergio; Rosati, Irma; Zedda, Maria Teresa; Pau, Salvatore; Bogliolo, Luisa. - In: THERIOGENOLOGY. - ISSN 0093-691X. - 77:1(2012), pp. 46-52. [10.1016/j.theriogenology.2011.07.013]

The effect of okadaic acid on meiotic maturation of canine oocytes of different size

BEBBERE, Daniela;LEDDA, Sergio;ROSATI, Irma;ZEDDA, Maria Teresa;PAU, Salvatore;BOGLIOLO, Luisa
2012-01-01

Abstract

The present study was conducted to determine the effect of okadic acid (OA), a potent inhibitor of seronine/treonine 1 and 2A phosphatase, on meiotic resumption and progression in canine oocytes with different diameters. Cumulus-oocyte complexes were collected from ovaries of bitches at different oestrous phases. In Experiment 1, to determine the optimal concentration of OA (0.5 or 2 mu M), the oocytes were pre-incubated for 1, 3, and 20 h in TCM 199 supplemented with 20% SCE and thereafter cultured in the same medium without OA. In Experiment 2, the selected oocytes were divided into three groups according to their diameter: <110 mu m, 110-120 mu m, >120 mu m, and pre-incubated in OA 0.5 mu M for 1 h. Oocytes were cultured in vitro as previously described. After 72 h of IVM, in Experiment 1, significantly more oocytes reached MII stage with 0.5 mu M for 1 h (30.8% P<0.001%) for oocytes cultured in other OA condition and in control group. In Experiment 2, OA induced a significantly higher incidence of MII oocytes in the 110-120 mu m and >120 mu m groups (P<0.001) compared to control group, but a significantly higher proportion of the oocytes >120 mu m pre-incubated with OA progressed to MII (51.3% P<0.001). In contrast, smaller oocytes (<110) did not develop to MII stage with or without OA. In conclusion, treatment of canine oocytes with 0.5 mu M for 1 h, improves meiotic maturation. The culture of fully grown (>120 mu m) oocytes with OA at the onset of in vitro maturation can result in a higher frequency of meiotic maturation. (C) 2012 Elsevier Inc. All rights reserved.
2012
The effect of okadaic acid on meiotic maturation of canine oocytes of different size / Ariu, F; Fois, S; Bebbere, Daniela; Ledda, Sergio; Rosati, Irma; Zedda, Maria Teresa; Pau, Salvatore; Bogliolo, Luisa. - In: THERIOGENOLOGY. - ISSN 0093-691X. - 77:1(2012), pp. 46-52. [10.1016/j.theriogenology.2011.07.013]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/62109
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 7
social impact