A method for the determination in vivo of the benzylpenicillin residues in ovine milk at low levels was described. Two groups of Sardinian breed sheep were treated with a dose of penicillin G sodium salt by intramammary infusion and intramuscular administration respectively. The residues were detected by isocratic HPLC method of the extracts obtained from a previous cleanup procedure. Linear calibration plots were obtained over a large concentration range of 1 mg ml1–10 ng ml1, with correlation coefficients greater than 0.998. Recoveries between 78.6 and 87.3% were obtained. Limit of detection (LOD) and limit of determination (LOQ) were 2.6 and 8.8 ng ml1 respectively. This method would be useful for routine monitoring of penicillin G residues in ovine dairy milk.
Monitoring of benzylpenicillin in ovine milk by HPLC / Boatto, Gianpiero; Cerri, R.; Pau, Amedeo; Palomba, M.; Pintore, Giorgio Antonio Mario; Denti, M. G.. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 17:4-5(1998), pp. 733-738. [10.1016/S0731-7085(97)00245-8]
Monitoring of benzylpenicillin in ovine milk by HPLC
BOATTO, Gianpiero;PAU, Amedeo;M. PALOMBA;PINTORE, Giorgio Antonio Mario;
1998-01-01
Abstract
A method for the determination in vivo of the benzylpenicillin residues in ovine milk at low levels was described. Two groups of Sardinian breed sheep were treated with a dose of penicillin G sodium salt by intramammary infusion and intramuscular administration respectively. The residues were detected by isocratic HPLC method of the extracts obtained from a previous cleanup procedure. Linear calibration plots were obtained over a large concentration range of 1 mg ml1–10 ng ml1, with correlation coefficients greater than 0.998. Recoveries between 78.6 and 87.3% were obtained. Limit of detection (LOD) and limit of determination (LOQ) were 2.6 and 8.8 ng ml1 respectively. This method would be useful for routine monitoring of penicillin G residues in ovine dairy milk.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
