In this paper we report the results of an investigation into the presence of enteric viruses in shellfish from the waters around Sardinia. Twenty two samples of shellfish were examined using a rapid and sensitive technique to concentrate and detect viral RNA in shellfish tissues. After recovery of viral particles, RNA was extracted, transcribed into cDNA and amplified using "nested PCR". Testing with enterovirus-specific RT-PCR produced positive results in over 13% of specimens. The virus detection procedure appears to be effective. In some circumstances it could be a better test of water quality than conventional monitoring techniques.
A molecular method for the recovery and identification of enteric virus in shellfish / Zanetti, Stefania Anna Lucia; Deriu, A; Manzara, S; Cattani, P; Mura, A; Molicotti, Paola; Fadda, G; Sechi, Leonardo Antonio. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 26:(2003), pp. 157-162.
A molecular method for the recovery and identification of enteric virus in shellfish
ZANETTI, Stefania Anna Lucia;MOLICOTTI, Paola;SECHI, Leonardo Antonio
2003-01-01
Abstract
In this paper we report the results of an investigation into the presence of enteric viruses in shellfish from the waters around Sardinia. Twenty two samples of shellfish were examined using a rapid and sensitive technique to concentrate and detect viral RNA in shellfish tissues. After recovery of viral particles, RNA was extracted, transcribed into cDNA and amplified using "nested PCR". Testing with enterovirus-specific RT-PCR produced positive results in over 13% of specimens. The virus detection procedure appears to be effective. In some circumstances it could be a better test of water quality than conventional monitoring techniques.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
