The in vitro activities of commercial Melaleuca alternifolia essential oil (tea tree oil; TTO) and chitosan were investigated against Candida albicans, both alone and in combination, to assess their potential synergistic action; their anticandidal properties were assessed using standard microbiological techniques, such as MIC evaluation and a ‘time–kill’ test. Our results demonstrated that both TTO and chitosan possess remarkable activity against Candida: TTO inhibited C. albicans at 0.5 % v/v and was able to kill yeast cells in 120min at 1% and in 300 min at 0.5–0.25%; as far as chitosan was concerned, 0.5 mg/ml were required to inhibit Candida growth in liquid medium, whereas 0.5–2 mg/ml chitosan required 24 h to kill them in the ‘time–kill’ test; this effect was pH-dependent, being present at pH 5.0 and disappearing at pH 2.0. When TTO and chitosan were tested together, the polymer reduced the MIC of TTO but did not enhance the activity of the oil as evaluated in the ‘time–kill’ test.
In vitro study on the anticandidal activity of Melaleuca alternifolia (tea tree) essential oil combined with chitosan / Juliano, Claudia Clelia Assunta; Demurtas, C; Piu, L.. - In: FLAVOUR AND FRAGRANCE JOURNAL. - ISSN 0882-5734. - 23:(2008), pp. 227-231. [10.1002/ffj.1871]
In vitro study on the anticandidal activity of Melaleuca alternifolia (tea tree) essential oil combined with chitosan
JULIANO, Claudia Clelia Assunta;
2008-01-01
Abstract
The in vitro activities of commercial Melaleuca alternifolia essential oil (tea tree oil; TTO) and chitosan were investigated against Candida albicans, both alone and in combination, to assess their potential synergistic action; their anticandidal properties were assessed using standard microbiological techniques, such as MIC evaluation and a ‘time–kill’ test. Our results demonstrated that both TTO and chitosan possess remarkable activity against Candida: TTO inhibited C. albicans at 0.5 % v/v and was able to kill yeast cells in 120min at 1% and in 300 min at 0.5–0.25%; as far as chitosan was concerned, 0.5 mg/ml were required to inhibit Candida growth in liquid medium, whereas 0.5–2 mg/ml chitosan required 24 h to kill them in the ‘time–kill’ test; this effect was pH-dependent, being present at pH 5.0 and disappearing at pH 2.0. When TTO and chitosan were tested together, the polymer reduced the MIC of TTO but did not enhance the activity of the oil as evaluated in the ‘time–kill’ test.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.