Mycoplasma agalactiae, the causative agent of contagious agalactia in small ruminants, produces a protein, named P80, that is detectable in all wild-type isolates examined to date and that appears expressed during the early phase of infection. We describe here the identification, cloning and expression of the gene encoding P80 (ma-mp81). The deduced amino acid sequence is consistent with a hydrophobic and basic protein that possesses a lipoprotein signal peptide. Sequence analysis of gene ma-mp81 suggests that P80 is a membrane lipoprotein that shows significant homology with other putative lipoproteins of M. pneumoniae. An internal 1-kb fragment of ma-mp81 was expressed in Escherichia coli as a 6XHis-tagged protein. The purified recombinant protein greatly reacted with polyclonal anti-P80 sera raised in lamb. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Sequence, cloning, expression and characterisation of the 81-kDa surface membrane protein (P80) of Mycoplasma agalactiae / Tola, S; Crobeddu, S; Chessa, G; Uzzau, Sergio; Idini, G; Ibba, B; Rocca, Stefano. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 202:1(2001), pp. 45-50. [10.1111/j.1574-6968.2001.tb10778.x]

Sequence, cloning, expression and characterisation of the 81-kDa surface membrane protein (P80) of Mycoplasma agalactiae

UZZAU, Sergio;ROCCA, Stefano
2001-01-01

Abstract

Mycoplasma agalactiae, the causative agent of contagious agalactia in small ruminants, produces a protein, named P80, that is detectable in all wild-type isolates examined to date and that appears expressed during the early phase of infection. We describe here the identification, cloning and expression of the gene encoding P80 (ma-mp81). The deduced amino acid sequence is consistent with a hydrophobic and basic protein that possesses a lipoprotein signal peptide. Sequence analysis of gene ma-mp81 suggests that P80 is a membrane lipoprotein that shows significant homology with other putative lipoproteins of M. pneumoniae. An internal 1-kb fragment of ma-mp81 was expressed in Escherichia coli as a 6XHis-tagged protein. The purified recombinant protein greatly reacted with polyclonal anti-P80 sera raised in lamb. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Sequence, cloning, expression and characterisation of the 81-kDa surface membrane protein (P80) of Mycoplasma agalactiae / Tola, S; Crobeddu, S; Chessa, G; Uzzau, Sergio; Idini, G; Ibba, B; Rocca, Stefano. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 202:1(2001), pp. 45-50. [10.1111/j.1574-6968.2001.tb10778.x]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/49260
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