The inverse PCR technique was applied to clone genomic DNA flanking insertion sites of sequences homologous to the transposable element Fot1 in the genome of a new pathogenic isolate of Fusarium oxysporum obtained from wilted Argyranthemum frutescens (Paris daisy). Based on the genomic flanking regions, a primer was designed which when paired to a second primer matching the Fot1 sequence allowed detection of this pathogen by PCR. The primer pair Mg5/Mg6 could specifically identify nine tested isolates of F. oxysporum from A. frutescens, when fungal genomic DNA was used as template. Moreover, the primer pair Mg5/Mg6 allowed successful detection of the pathogen in stem and root tissue from asymptomatic plants that were artificially inoculated with a representative isolate of F. oxysporum from A. frutescens.

Development of PCR primers for a new Fusarium oxysporum pathogenic on Paris daisy (Argyranthemum frutescens L.) / Pasquali, M; Acquadro, A; Balmas, Virgilio; Migheli, Quirico; Gullino, Ml; Garibaldi, A.. - In: EUROPEAN JOURNAL OF PLANT PATHOLOGY. - ISSN 0929-1873. - 110:1(2004), pp. 7-11. [10.1023/B:EJPP.0000010141.37327.d0]

Development of PCR primers for a new Fusarium oxysporum pathogenic on Paris daisy (Argyranthemum frutescens L.)

BALMAS, Virgilio;MIGHELI, Quirico;
2004

Abstract

The inverse PCR technique was applied to clone genomic DNA flanking insertion sites of sequences homologous to the transposable element Fot1 in the genome of a new pathogenic isolate of Fusarium oxysporum obtained from wilted Argyranthemum frutescens (Paris daisy). Based on the genomic flanking regions, a primer was designed which when paired to a second primer matching the Fot1 sequence allowed detection of this pathogen by PCR. The primer pair Mg5/Mg6 could specifically identify nine tested isolates of F. oxysporum from A. frutescens, when fungal genomic DNA was used as template. Moreover, the primer pair Mg5/Mg6 allowed successful detection of the pathogen in stem and root tissue from asymptomatic plants that were artificially inoculated with a representative isolate of F. oxysporum from A. frutescens.
Development of PCR primers for a new Fusarium oxysporum pathogenic on Paris daisy (Argyranthemum frutescens L.) / Pasquali, M; Acquadro, A; Balmas, Virgilio; Migheli, Quirico; Gullino, Ml; Garibaldi, A.. - In: EUROPEAN JOURNAL OF PLANT PATHOLOGY. - ISSN 0929-1873. - 110:1(2004), pp. 7-11. [10.1023/B:EJPP.0000010141.37327.d0]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/48774
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