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EnglishThe inverse PCR technique was applied to clone genomic DNA flanking insertion sites of sequences homologous to the transposable element Fot1 in the genome of a new pathogenic isolate of Fusarium oxysporum obtained from wilted Argyranthemum frutescens (Paris daisy). Based on the genomic flanking regions, a primer was designed which when paired to a second primer matching the Fot1 sequence allowed detection of this pathogen by PCR. The primer pair Mg5/Mg6 could specifically identify nine tested isolates of F. oxysporum from A. frutescens, when fungal genomic DNA was used as template. Moreover, the primer pair Mg5/Mg6 allowed successful detection of the pathogen in stem and root tissue from asymptomatic plants that were artificially inoculated with a representative isolate of F. oxysporum from A. frutescens.
Development of PCR primers for a new Fusarium oxysporum pathogenic on Paris daisy (Argyranthemum frutescens L.) / Pasquali M; Acquadro A; Balmas V; Migheli Q; Gullino ML; Garibaldi A. - In: EUROPEAN JOURNAL OF PLANT PATHOLOGY. - ISSN 0929-1873. - 110:1(2004), pp. 7-11. [10.1023/B:EJPP.0000010141.37327.d0]
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| Titolo: | Development of PCR primers for a new Fusarium oxysporum pathogenic on Paris daisy (Argyranthemum frutescens L.) | |
| Autori: | ||
| Data di pubblicazione: | 2004 | |
| Rivista: | ||
| Citazione: | Development of PCR primers for a new Fusarium oxysporum pathogenic on Paris daisy (Argyranthemum frutescens L.) / Pasquali M; Acquadro A; Balmas V; Migheli Q; Gullino ML; Garibaldi A. - In: EUROPEAN JOURNAL OF PLANT PATHOLOGY. - ISSN 0929-1873. - 110:1(2004), pp. 7-11. [10.1023/B:EJPP.0000010141.37327.d0] | |
| Abstract: | The inverse PCR technique was applied to clone genomic DNA flanking insertion sites of sequences homologous to the transposable element Fot1 in the genome of a new pathogenic isolate of Fusarium oxysporum obtained from wilted Argyranthemum frutescens (Paris daisy). Based on the genomic flanking regions, a primer was designed which when paired to a second primer matching the Fot1 sequence allowed detection of this pathogen by PCR. The primer pair Mg5/Mg6 could specifically identify nine tested isolates of F. oxysporum from A. frutescens, when fungal genomic DNA was used as template. Moreover, the primer pair Mg5/Mg6 allowed successful detection of the pathogen in stem and root tissue from asymptomatic plants that were artificially inoculated with a representative isolate of F. oxysporum from A. frutescens. | |
| Handle: | http://hdl.handle.net/11388/48774 | |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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