OBJECTIVE To evaluate the effects of phytocomplexes of Uncaria, Shitake and Ribes in terms of viability and inflammatory response on immune cell-derived cultures. METHODS Standardized extracts of Uncaria, Shitake and Ribes and their commercial formulation were tested on cell lines PBMC, U937 and macrophage,. The activity was evaluated in terms of cell viability (MTT test), variations of oxidative markers release (ROS and PGE2) and modulatory effects on immune response (RT-PCR expression of IL-6, IL-8 and TNF). Page 2 of 19 PEER REVIEW COPY EDIZIONI MINERVA MEDICA Minerva Medica 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 RESULTS Cell viability was not affected by extracts, except subtle variations observed only at higher doses (>250Hg/ml). The extract mixture was well tolerated, with no effects on cell viability up to doses of 500 μg/ml. Pre-treatment of macrophages with subtoxic doses of the extracts reduced the basal release of oxidative markers and enhanced the cell response to oxogenus oxidant stimulation, as revealed by ROS and PGE2 release reduction. The same treatment on macrophage resulted in a selective modulation of the immune response, as shown by an increase of IL-6 mRNA and, partially, IL-8 mRNA, while a reduction was observed for TNF mRNA. Data confirm that extracts and their formulations can act as regulator of the immune system with mechanisms involving the oxidative stress and the release of selected proinflammatory cytokines.
A multiherbal formulation influencing immune response in vitro / L., Menghini; L., Leporini; N., Scanu; Pintore, Giorgio Antonio Mario; C., Ferrante; L., Recinella; G., Orlando; M., Vacca; L., Brunetti. - In: MINERVA MEDICA. - ISSN 0026-4806. - 103:1(2012), pp. 13-21.
A multiherbal formulation influencing immune response in vitro
PINTORE, Giorgio Antonio Mario;
2012-01-01
Abstract
OBJECTIVE To evaluate the effects of phytocomplexes of Uncaria, Shitake and Ribes in terms of viability and inflammatory response on immune cell-derived cultures. METHODS Standardized extracts of Uncaria, Shitake and Ribes and their commercial formulation were tested on cell lines PBMC, U937 and macrophage,. The activity was evaluated in terms of cell viability (MTT test), variations of oxidative markers release (ROS and PGE2) and modulatory effects on immune response (RT-PCR expression of IL-6, IL-8 and TNF). Page 2 of 19 PEER REVIEW COPY EDIZIONI MINERVA MEDICA Minerva Medica 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 RESULTS Cell viability was not affected by extracts, except subtle variations observed only at higher doses (>250Hg/ml). The extract mixture was well tolerated, with no effects on cell viability up to doses of 500 μg/ml. Pre-treatment of macrophages with subtoxic doses of the extracts reduced the basal release of oxidative markers and enhanced the cell response to oxogenus oxidant stimulation, as revealed by ROS and PGE2 release reduction. The same treatment on macrophage resulted in a selective modulation of the immune response, as shown by an increase of IL-6 mRNA and, partially, IL-8 mRNA, while a reduction was observed for TNF mRNA. Data confirm that extracts and their formulations can act as regulator of the immune system with mechanisms involving the oxidative stress and the release of selected proinflammatory cytokines.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
