Neopterin, a pyrazinopyrimidine compound, serves as a marker of cellular immune system activation, and it can be used as a prognostic predictor for certain types of diseases. We propose a new simple HPLC method to measure serum neopterin with highly sensitive fluorimetric detection. After TCA serum protein precipitation, the supernatant was diluted five times, injected into a C-18 reversed-phase column and eluted at a flow rate of 1.5 mL/min by an isocratic water-acetonitrile (99:1) mobile phase. The natural fluorescence of the molecule was detected at excitation wavelength 353 nm and emission 438 nm. In these conditions the neopterin retention time was about 4 min. Our proposed method was compared with a validated chromatographic separation, and the obtained data of the serum neopterin from 35 healthy volunteers were analysed by Passing-Bablok regression and Bland-Altman test. Neopterin measurement in healthy subjects was also employed to investigate on its potential relationships with plasma thiols levels. Copyright (C) 2004 John Wiley Sons, Ltd.
A new HPLC method for serum neopterin measurement and relationships with plasma thiols levels in healthy subjects / Carru, Ciriaco; Zinellu, Angelo; Sotgia, S; Serra, R; Usai, Mf; Pintus, Gianfranco; Pes, Giovanni Mario; Deiana, Luca. - In: BIOMEDICAL CHROMATOGRAPHY. - ISSN 0269-3879. - 18:6(2004), pp. 360-366. [10.1002/bmc.325]