Overexpression of protein kinase C-delta and protein kinase C-delta has been shown to modulate a number of biological effects, including the cell growth and differentiation. We hypothesized that heparin, a potent antimitogenic drug, could affect the cell proliferation by inhibiting the expression of specific protein kinase C genes. Heparin, markedly but not completely, inhibited the serum-stimulated protein kinase C-delta and -delta mRNA expression. Protein kinase C inhibition or downregulation significantly decreased the serum-induced protein kinase C isoenzyme gene expression. Heparin failed to inhibit the residual effect of serum that was resistant to the above-mentioned treatments. Phorbol 12-myristate 13-acetate elicited an increase of protein kinase C isoenzyme gene expression that was completely prevented by protein kinase C inhibition or downregulation. Heparin dose-dependently counteracted and ultimately abolished the increase in the protein kinase C isoenzyme gene expression elicited by phorbol 12-myristate 13-acetate, These results suggest that the inhibition of an autoregulatory role wielded by protein kinase C on the protein kinase C-delta and -delta gene expression might represent a possible mechanism by which glycosaminoglycans modulate the cell growth. (C) 1999 Federation of European Biochemical Societies.

Heparin down-regulates the phorbol ester-induced protein kinase C gene expression in human endothelial cells: enzyme-mediated autoregulation of protein kinase C-alpha and -delta genes / Pintus, Gianfranco; Tadolini, B; Maioli, Margherita; Posadino, Am; Gaspa, Leonardo; Ventura, C.. - In: FEBS LETTERS. - ISSN 0014-5793. - 449:2-3(1999), pp. 135-140. [10.1016/S0014-5793(99)00438-X]

Heparin down-regulates the phorbol ester-induced protein kinase C gene expression in human endothelial cells: enzyme-mediated autoregulation of protein kinase C-alpha and -delta genes

PINTUS, Gianfranco;MAIOLI, Margherita;POSADINO AM;GASPA, Leonardo;
1999-01-01

Abstract

Overexpression of protein kinase C-delta and protein kinase C-delta has been shown to modulate a number of biological effects, including the cell growth and differentiation. We hypothesized that heparin, a potent antimitogenic drug, could affect the cell proliferation by inhibiting the expression of specific protein kinase C genes. Heparin, markedly but not completely, inhibited the serum-stimulated protein kinase C-delta and -delta mRNA expression. Protein kinase C inhibition or downregulation significantly decreased the serum-induced protein kinase C isoenzyme gene expression. Heparin failed to inhibit the residual effect of serum that was resistant to the above-mentioned treatments. Phorbol 12-myristate 13-acetate elicited an increase of protein kinase C isoenzyme gene expression that was completely prevented by protein kinase C inhibition or downregulation. Heparin dose-dependently counteracted and ultimately abolished the increase in the protein kinase C isoenzyme gene expression elicited by phorbol 12-myristate 13-acetate, These results suggest that the inhibition of an autoregulatory role wielded by protein kinase C on the protein kinase C-delta and -delta gene expression might represent a possible mechanism by which glycosaminoglycans modulate the cell growth. (C) 1999 Federation of European Biochemical Societies.
1999
Heparin down-regulates the phorbol ester-induced protein kinase C gene expression in human endothelial cells: enzyme-mediated autoregulation of protein kinase C-alpha and -delta genes / Pintus, Gianfranco; Tadolini, B; Maioli, Margherita; Posadino, Am; Gaspa, Leonardo; Ventura, C.. - In: FEBS LETTERS. - ISSN 0014-5793. - 449:2-3(1999), pp. 135-140. [10.1016/S0014-5793(99)00438-X]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/45574
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