Severe damage caused by Pseudomonas corrugate Roberts et Scarlett on tornato soilless grown. Severe damage caused by Pseudomonas Roberts et Scarlett occurred, on tomato cultivated by New Growing System (NGS), in greenhouse in Sardinia. Early simptoms consisted in yellowing of the lower leaves, adventious root formation, root necrosis, vascular discoloration and pith necrosis. Infected plants collapsed, wilted and died J'seudomonas corrugata was isolated from plants and from nutrient circulating solution. Pathological, biochemical and serological tests on isolated strains confirmed that isolates belong to P. corrugata. Polymerase chain reaction (PCR) performed by using specific primes, revealed the presence of either one of two bands (600 bp and 1100 bp) typical of corrugata, representing I and I I groups. An atypical band obtained from a sardinian strain IPV-SS6FP suggest the presence of an insertion sequence of 20-50 bp. ELISA results on infected plants and nutrient circulating solution probably rely on bacterial concentration. Suitable control means to prevent or contain this disease are suggested.
Gravi attacchi di Pseudomonas corrugata Roberts et Scarlett su pomodoro coltivato fuori suolo / Fiori, Mario. - In: INFORMATORE FITOPATOLOGICO. - ISSN 0020-0735. - 52:1(2002), pp. 47-51.
Gravi attacchi di Pseudomonas corrugata Roberts et Scarlett su pomodoro coltivato fuori suolo
FIORI, Mario
2002-01-01
Abstract
Severe damage caused by Pseudomonas corrugate Roberts et Scarlett on tornato soilless grown. Severe damage caused by Pseudomonas Roberts et Scarlett occurred, on tomato cultivated by New Growing System (NGS), in greenhouse in Sardinia. Early simptoms consisted in yellowing of the lower leaves, adventious root formation, root necrosis, vascular discoloration and pith necrosis. Infected plants collapsed, wilted and died J'seudomonas corrugata was isolated from plants and from nutrient circulating solution. Pathological, biochemical and serological tests on isolated strains confirmed that isolates belong to P. corrugata. Polymerase chain reaction (PCR) performed by using specific primes, revealed the presence of either one of two bands (600 bp and 1100 bp) typical of corrugata, representing I and I I groups. An atypical band obtained from a sardinian strain IPV-SS6FP suggest the presence of an insertion sequence of 20-50 bp. ELISA results on infected plants and nutrient circulating solution probably rely on bacterial concentration. Suitable control means to prevent or contain this disease are suggested.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
