We have developed a Real-Time PCR assay to detect M. tuberculosis using the iCycler iQ detection system by TaqMan assay directly on the clinical specimen. A total of 513 clinical samples were taken from patients with suspected tuberculosis and other patients that had an active mycobacterial infection, as well as patients with diagnosed tuberculosis who were receiving antitubercular therapy. The sensitivity and specificity of this assay, 10% and 100%, respectively, were compared to those of conventional microbiological methods.
We have developed a Real-Time PCR assay to detect M. tuberculosis using the iCycler iQ detection system by TaqMan assay directly on the clinical specimen. A total of 513 clinical samples were taken from patients with suspected tuberculosis and other patients that had an active mycobacterial infection, as well as patients with diagnosed tuberculosis who were receiving antitubercular therapy. The sensitivity and specificity of this assay, 10% and 100%, respectively, were compared to those of conventional microbiological methods.
Rapid detection and identification of Mycobacterium tuberculosis by Real Time PCR and Bactec 960 MIGT / Ortu, Silvia; Molicotti, Paola; Sechi, Leonardo Antonio; Pirina, Pietro; Saba, F; Vertuccio, C; Deriu, A; Maida, Ivana; Mura, Maria Stella Anna; Zanetti, S.. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 29:(2006), pp. 75-80.
Rapid detection and identification of Mycobacterium tuberculosis by Real Time PCR and Bactec 960 MIGT
ORTU, Silvia;MOLICOTTI, Paola;SECHI, Leonardo Antonio;PIRINA, Pietro;MAIDA, Ivana;MURA, Maria Stella Anna;
2006-01-01
Abstract
We have developed a Real-Time PCR assay to detect M. tuberculosis using the iCycler iQ detection system by TaqMan assay directly on the clinical specimen. A total of 513 clinical samples were taken from patients with suspected tuberculosis and other patients that had an active mycobacterial infection, as well as patients with diagnosed tuberculosis who were receiving antitubercular therapy. The sensitivity and specificity of this assay, 10% and 100%, respectively, were compared to those of conventional microbiological methods.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
