• Background: In the present research, we examined the influence of the dietary inclusion of Hermetia illucens larvae meal on the diversity and composition of the bacterial community in the caecum of Barbary partridges (Alectoris barbara). A total of 54 partridges were divided equally into three treatment groups. The control group (C) received a diet containing corn-soybean meal and the two experimental groups received diets in which soybean meal protein was partially substituted by H. illucens larvae meal in a proportion of 25% (H25) and 50% (H50), respectively. The bacterial community of the caecal samples of 30 (10 per group) slaughtered animals at 64 days of age was investigated by high-throughput sequencing using the V4-V5 region of the 16S rRNA gene. • Results: Firmicutes was the most abundant phylum in all studied categories, this phylum was dominated by families of Ruminococcaceae and Lachnospiraceae. The caecal microbiota had been significantly altered at the genus level. The linear discriminant analysis effect size (LefSe) analysis for the differential taxa abundance revealed several statistical dissimilarities between the control group (C) and the groups with 25% and 50% insect meal replacement, showing 13 and 20 significantly different taxa at the genus level, respectively. The partridges fed 25% H. illucens meal had a considerably higher bacterial phylogenetic abundance and richness than the control group. The beta diversity measurements showed that all three groups of animals were significantly spatially separated. • Conclusions: The findings demonstrated the significant impact of black soldier fly larvae meal on Barbary partridge’s caecal microbiota, showing a positive influence of used insect meal as indicated by increased bacterial diversity in the H25 group and increased relative abundance of several potentially beneficial genera in both experimental groups.

The effect of different levels of Hermetia illucens oil inclusion on caecal microbiota of Japanese quails (Coturnix japonica, Gould, 1837) / Atallah, E.; Mahayri, T. M.; Fliegerová, K. O.; Mrázek, J.; Addeo, N. F.; Bovera, F.; Moniello, G.. - In: JOURNAL OF INSECTS AS FOOD AND FEED. - ISSN 2352-4588. - 10:1(2024), pp. -189. [10.1163/23524588-20230052]

The effect of different levels of Hermetia illucens oil inclusion on caecal microbiota of Japanese quails (Coturnix japonica, Gould, 1837)

Atallah, E.;Mahayri, T. M.;Moniello, G.
2024-01-01

Abstract

• Background: In the present research, we examined the influence of the dietary inclusion of Hermetia illucens larvae meal on the diversity and composition of the bacterial community in the caecum of Barbary partridges (Alectoris barbara). A total of 54 partridges were divided equally into three treatment groups. The control group (C) received a diet containing corn-soybean meal and the two experimental groups received diets in which soybean meal protein was partially substituted by H. illucens larvae meal in a proportion of 25% (H25) and 50% (H50), respectively. The bacterial community of the caecal samples of 30 (10 per group) slaughtered animals at 64 days of age was investigated by high-throughput sequencing using the V4-V5 region of the 16S rRNA gene. • Results: Firmicutes was the most abundant phylum in all studied categories, this phylum was dominated by families of Ruminococcaceae and Lachnospiraceae. The caecal microbiota had been significantly altered at the genus level. The linear discriminant analysis effect size (LefSe) analysis for the differential taxa abundance revealed several statistical dissimilarities between the control group (C) and the groups with 25% and 50% insect meal replacement, showing 13 and 20 significantly different taxa at the genus level, respectively. The partridges fed 25% H. illucens meal had a considerably higher bacterial phylogenetic abundance and richness than the control group. The beta diversity measurements showed that all three groups of animals were significantly spatially separated. • Conclusions: The findings demonstrated the significant impact of black soldier fly larvae meal on Barbary partridge’s caecal microbiota, showing a positive influence of used insect meal as indicated by increased bacterial diversity in the H25 group and increased relative abundance of several potentially beneficial genera in both experimental groups.
2024
The effect of different levels of Hermetia illucens oil inclusion on caecal microbiota of Japanese quails (Coturnix japonica, Gould, 1837) / Atallah, E.; Mahayri, T. M.; Fliegerová, K. O.; Mrázek, J.; Addeo, N. F.; Bovera, F.; Moniello, G.. - In: JOURNAL OF INSECTS AS FOOD AND FEED. - ISSN 2352-4588. - 10:1(2024), pp. -189. [10.1163/23524588-20230052]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/322536
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