Due to the high variability of data drawn from the literature, aim of the study was to evaluate the effects of exercise on creatine-kinase (CK) serum activity and to determine CK reference intervals (RIs) at rest and post-exercise in healthy Standardbred racehorses. Data concerning history, physical examination, laboratory evaluation and ECG were collected retrospectively from a population of 258 Standardbred racehorses in training that underwent an incremental-maximal treadmill exercise. Those subjects with alterations potentially influencing CK serum activity were excluded. Finally, a reference sample of 194 horses was selected. Blood samples were collected 1 hour before exercise and 6 hour post-exercise and analysed with a spectrophotometric method. Values were compared by Wilcoxon test for paired samples. The effect of age and sex was evaluated by Kruskal-Wallis and Dunn post-test. Statistical significance was set at P<0.05. RIs were determined following Clinical and Laboratory Standard Institute guidelines (CLSI), approved by the American Society for Veterinary Clinical Pathology. Using a macroinstruction set for Microsoft Excel (RefValAdv), RIs were determined with a non-parametric method. A significant increase (P<0.0001) in CK activity post-exercise was observed. Partition by sex and age did not show any statistical difference, either at rest or post-exercise. In RIs determination no outliers were identified. RIs ranged from 25 to 394 U/l at rest and from 44 to 735 U/l post-exercise. To our knowledge, this is the first study considering CK post-exercise RIs in racehorses using CLSI's guidelines and specific CK-related exclusion criteria. These RIs could be useful to discriminate between physiological and pathological CK post-exercise increase.

Creatine-kinase reference intervals at rest and after maximal exercise in Standardbred racehorses / Stucchi, L.; Valli, C.; Stancari, G.; Zucca, E.; Ferrucci, F.. - In: COMPARATIVE EXERCISE PHYSIOLOGY. - ISSN 1755-2540. - 15:5(2019), pp. 319-325. [10.3920/CEP190020]

Creatine-kinase reference intervals at rest and after maximal exercise in Standardbred racehorses

Stucchi L.;
2019-01-01

Abstract

Due to the high variability of data drawn from the literature, aim of the study was to evaluate the effects of exercise on creatine-kinase (CK) serum activity and to determine CK reference intervals (RIs) at rest and post-exercise in healthy Standardbred racehorses. Data concerning history, physical examination, laboratory evaluation and ECG were collected retrospectively from a population of 258 Standardbred racehorses in training that underwent an incremental-maximal treadmill exercise. Those subjects with alterations potentially influencing CK serum activity were excluded. Finally, a reference sample of 194 horses was selected. Blood samples were collected 1 hour before exercise and 6 hour post-exercise and analysed with a spectrophotometric method. Values were compared by Wilcoxon test for paired samples. The effect of age and sex was evaluated by Kruskal-Wallis and Dunn post-test. Statistical significance was set at P<0.05. RIs were determined following Clinical and Laboratory Standard Institute guidelines (CLSI), approved by the American Society for Veterinary Clinical Pathology. Using a macroinstruction set for Microsoft Excel (RefValAdv), RIs were determined with a non-parametric method. A significant increase (P<0.0001) in CK activity post-exercise was observed. Partition by sex and age did not show any statistical difference, either at rest or post-exercise. In RIs determination no outliers were identified. RIs ranged from 25 to 394 U/l at rest and from 44 to 735 U/l post-exercise. To our knowledge, this is the first study considering CK post-exercise RIs in racehorses using CLSI's guidelines and specific CK-related exclusion criteria. These RIs could be useful to discriminate between physiological and pathological CK post-exercise increase.
2019
Creatine-kinase reference intervals at rest and after maximal exercise in Standardbred racehorses / Stucchi, L.; Valli, C.; Stancari, G.; Zucca, E.; Ferrucci, F.. - In: COMPARATIVE EXERCISE PHYSIOLOGY. - ISSN 1755-2540. - 15:5(2019), pp. 319-325. [10.3920/CEP190020]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/319282
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