: Tyrosinase is a well‐known copper‐containing metalloenzyme typically involved in the synthesis of melanin. Recently, curcumin and several synthetic derivatives have been recognized as tyrosinase inhibitors with interesting anti‐melanogenic therapeutic activity. In this study, three curcumin‐inspired compounds 1, 6 and 7 were prepared in yields ranging from 60 to 88 % and spectrophotometric, electrochemical, in vitro and in silico analyses were carried out. The viability of PC12 cells, a rat pheochromocytoma derived‐cell line, with compounds 1, 6 and 7, showed values around 80% at 5 μM concentration. In cell proliferation assays, compounds 1, 6 and 7 did not show significant toxicity on fibroblasts nor melanoma cells up to 10 μM with viability values over 90%. The inhibition of tyrosinase activity was evaluated both by a UV‐Vis spectroscopic method at two different concentrations, 0.2 and 2.0 μM, and by amperometric assay with IC50 for compounds 1, 6 and 7 ranging from 11 to 24 nM. Melanin content assays on human melanoma cells were performed to test the capability of compounds to inhibit melanin biosynthesis. All compounds exerted a decrease in melanin content, with compound 7 being the most effective by showing a melanogenesis inhibition up to four times greater than arbutin at 100 μM. Moreover, the antioxidant activity of the selected inhibitors was evaluated against H2O2 in amperometric experiments, whereby compound 7 was about three times more effective compared to compounds 1 and 6. The tyrosinase X‐ray structure of Bacterium megaterium crystal was used to carry out molecular docking studies in the presence of compounds 1, 6 and 7 in comparison with that of kojic acid and arbutin, two conventional tyrosinase inhibitors. Molecular docking of compounds 6 and 7 confirmed the high affinity of these compounds to tyrosinase protein

Inhibitory Effect of Curcumin‐Inspired Derivatives on Tyrosinase Activity and Melanogenesis / Rocchitta, Gaia Giovanna Maria; Rozzo, Carla; Pisano, Marina; Fabbri, Davide; Antonietta Dettori, Maria; Ruzza, Paolo; Honisch, Claudia; Dallocchio, Roberto; Dessì, Alessandro; Migheli, Rossana; Serra, Pier Andrea; Delogu, Giovanna Maria. - In: MOLECULES. - ISSN 1420-3049. - 27:22(2022), p. 7942. [10.3390/ molecules27227942]

Inhibitory Effect of Curcumin‐Inspired Derivatives on Tyrosinase Activity and Melanogenesis

Gaia Rocchitta;Rossana Migheli;Pier Andrea Serra;Giovanna Delogu
2022

Abstract

: Tyrosinase is a well‐known copper‐containing metalloenzyme typically involved in the synthesis of melanin. Recently, curcumin and several synthetic derivatives have been recognized as tyrosinase inhibitors with interesting anti‐melanogenic therapeutic activity. In this study, three curcumin‐inspired compounds 1, 6 and 7 were prepared in yields ranging from 60 to 88 % and spectrophotometric, electrochemical, in vitro and in silico analyses were carried out. The viability of PC12 cells, a rat pheochromocytoma derived‐cell line, with compounds 1, 6 and 7, showed values around 80% at 5 μM concentration. In cell proliferation assays, compounds 1, 6 and 7 did not show significant toxicity on fibroblasts nor melanoma cells up to 10 μM with viability values over 90%. The inhibition of tyrosinase activity was evaluated both by a UV‐Vis spectroscopic method at two different concentrations, 0.2 and 2.0 μM, and by amperometric assay with IC50 for compounds 1, 6 and 7 ranging from 11 to 24 nM. Melanin content assays on human melanoma cells were performed to test the capability of compounds to inhibit melanin biosynthesis. All compounds exerted a decrease in melanin content, with compound 7 being the most effective by showing a melanogenesis inhibition up to four times greater than arbutin at 100 μM. Moreover, the antioxidant activity of the selected inhibitors was evaluated against H2O2 in amperometric experiments, whereby compound 7 was about three times more effective compared to compounds 1 and 6. The tyrosinase X‐ray structure of Bacterium megaterium crystal was used to carry out molecular docking studies in the presence of compounds 1, 6 and 7 in comparison with that of kojic acid and arbutin, two conventional tyrosinase inhibitors. Molecular docking of compounds 6 and 7 confirmed the high affinity of these compounds to tyrosinase protein
Inhibitory Effect of Curcumin‐Inspired Derivatives on Tyrosinase Activity and Melanogenesis / Rocchitta, Gaia Giovanna Maria; Rozzo, Carla; Pisano, Marina; Fabbri, Davide; Antonietta Dettori, Maria; Ruzza, Paolo; Honisch, Claudia; Dallocchio, Roberto; Dessì, Alessandro; Migheli, Rossana; Serra, Pier Andrea; Delogu, Giovanna Maria. - In: MOLECULES. - ISSN 1420-3049. - 27:22(2022), p. 7942. [10.3390/ molecules27227942]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/298084
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