Four different normalisation techniques were applied for the corrections of fluorescence data generated by a cDNA microarray experiment. Correction for inaccurate signals and possible bias induced by fluorescence intensity, background intensity and dye effect were used in different combinations. Results of the present study highlight a pronounced role for the normalisation techniques in the absolute number of genes different expressed and a low concordance between different methods. Moreover, a significant effect of the dependent variable used, mean or median fluorescence intensity, was observed.
Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis / Macciotta, Nicolò Pietro Paolo; Bacciu, Nicola; Dimauro, Corrado. - 6:Suppl. 1(2007), pp. 122-124.
Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis
Macciotta, Nicolò Pietro Paolo;Dimauro, Corrado
2007-01-01
Abstract
Four different normalisation techniques were applied for the corrections of fluorescence data generated by a cDNA microarray experiment. Correction for inaccurate signals and possible bias induced by fluorescence intensity, background intensity and dye effect were used in different combinations. Results of the present study highlight a pronounced role for the normalisation techniques in the absolute number of genes different expressed and a low concordance between different methods. Moreover, a significant effect of the dependent variable used, mean or median fluorescence intensity, was observed.| File | Dimensione | Formato | |
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