In this study we present the real-time monitoring of three key brain neurochemical species in conscious rats using implantable amperometric electrodes interfaced to a biotelemetric device. The new system, derived from a previous design, was coupled with carbon-based microsensors and a platinum-based biosensor for the detection of ascorbic acid (AA), O2and glucose in the striatum of untethered, freely-moving rats. The miniaturized device consisted of a single-supply sensor driver, a current-to-voltage converter, a microcontroller and a miniaturized data transmitter. The redox currents were digitized to digital values by means of an analog-to-digital converter integrated in a peripheral interface controller (PIC), and sent to a personal computer by means of a miniaturized AM transmitter. The electronics were calibrated and testedin vitrounder different experimental conditions and exhibited high stability, low power consumption and good linear response in the nanoampere current range. Thein-vivoresults confirmed ON ACCESSpreviously published observations on striatal AA, oxygen and glucose dynamics recorded in tethered rats. This approach, based on simple and inexpensive components, could be used as a rapid and reliable model for studying the effects of different drugs on brain neurochemical systems.

Biotelemetric monitoring of brain neurochemistry in conscious rats using microsensors and biosensors / Migheli, Rossana; Mazzarello, Vittorio Lorenzo Giuseppe; Desole, Maria Speranza; Puggioni, Giulia Maria Grazia; Spissu, Ylenia; Lowry, John P.; O’Neill, Robert D.; Rocchitta, Gaia Giovanna Maria; Serra, Pier Andrea; Calia, Giammario; Bazzu, Gianfranco. - In: SENSORS. - ISSN 1424-8220. - 9:4(2009), pp. 2511-2523. [10.3390/s90402511]

Biotelemetric monitoring of brain neurochemistry in conscious rats using microsensors and biosensors

Migheli, Rossana;Mazzarello, Vittorio Lorenzo Giuseppe;Desole, Maria Speranza;Spissu, Ylenia;Rocchitta, Gaia Giovanna Maria;Serra, Pier Andrea;Calia, Giammario;Bazzu, Gianfranco
2009-01-01

Abstract

In this study we present the real-time monitoring of three key brain neurochemical species in conscious rats using implantable amperometric electrodes interfaced to a biotelemetric device. The new system, derived from a previous design, was coupled with carbon-based microsensors and a platinum-based biosensor for the detection of ascorbic acid (AA), O2and glucose in the striatum of untethered, freely-moving rats. The miniaturized device consisted of a single-supply sensor driver, a current-to-voltage converter, a microcontroller and a miniaturized data transmitter. The redox currents were digitized to digital values by means of an analog-to-digital converter integrated in a peripheral interface controller (PIC), and sent to a personal computer by means of a miniaturized AM transmitter. The electronics were calibrated and testedin vitrounder different experimental conditions and exhibited high stability, low power consumption and good linear response in the nanoampere current range. Thein-vivoresults confirmed ON ACCESSpreviously published observations on striatal AA, oxygen and glucose dynamics recorded in tethered rats. This approach, based on simple and inexpensive components, could be used as a rapid and reliable model for studying the effects of different drugs on brain neurochemical systems.
2009
Biotelemetric monitoring of brain neurochemistry in conscious rats using microsensors and biosensors / Migheli, Rossana; Mazzarello, Vittorio Lorenzo Giuseppe; Desole, Maria Speranza; Puggioni, Giulia Maria Grazia; Spissu, Ylenia; Lowry, John P.; O’Neill, Robert D.; Rocchitta, Gaia Giovanna Maria; Serra, Pier Andrea; Calia, Giammario; Bazzu, Gianfranco. - In: SENSORS. - ISSN 1424-8220. - 9:4(2009), pp. 2511-2523. [10.3390/s90402511]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/263906
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