Interferon (IFN)-γ release assays (IGRA) have improved tuberculosis contact tracing, but discrimination of recent from remoteMycobacterium tuberculosiscontacts is not possible by IGRA alone. We present results of a tuberculosis contact investigation with a new early-secretory-antigenic-target (ESAT)-6 and culture-filtrate-protein (CFP)-10 specific interleukin (IL)-2 ELISpot in addition to ESAT-6 and CFP-10 specific IFN-γ ELISpot and tuberculin skin testing (TST). Results of the TST, IFN-γ ELISpot and IL-2 ELISpot were positive in 6/172 (3.4%), 7/167 (4.2%) and 6/196 (3.1%) of contacts, respectively. Close contact (≥100 hours) to the index case increased the risk of positive results in the IFN-γ ELISpot, TST, and IL-2 ELISpot by 40.8, 19.3, and 2.5 times, respectively. Individuals with a positive IFN-γ ELISpot/negative IL-2 ELISpot result had a median (IQR) duration of index case exposure of 568 hours (133_1000) compared to individuals with a positive IFN-γ ELISpot/positive IL-2 ELISpot result (median=24 hours; 20_130; p-value=0.047). Combination of aM. tuberculosisspecific IFN-γ ELISpot with aM. tuberculosisspecific IL-2 ELISpot significantly improved the identification of individuals with the highest risk of recentM. tuberculosisinfection and is a promising method that should be explored to target tuberculosis preventive chemotherapy.
Potential role for IL-2 ELISpot in differentiating recent and remote infection in tuberculosis contact tracing / Sotgiu, Giovanni; Krummel, Benjamin; Strassburg, Alan; Ernst, Martin; Reiling, Norbert; Eker, Barbara; Rath, Heidrun; Wappler, Waltraud; Glaewe, Andrea; Lange, Christoph; Hörster, Robert; Schöllhorn, Volker. - In: PLOS ONE. - ISSN 1932-6203. - 5:7(2010). [10.1371/journal.pone.0011670]
Potential role for IL-2 ELISpot in differentiating recent and remote infection in tuberculosis contact tracing
Sotgiu, Giovanni;
2010-01-01
Abstract
Interferon (IFN)-γ release assays (IGRA) have improved tuberculosis contact tracing, but discrimination of recent from remoteMycobacterium tuberculosiscontacts is not possible by IGRA alone. We present results of a tuberculosis contact investigation with a new early-secretory-antigenic-target (ESAT)-6 and culture-filtrate-protein (CFP)-10 specific interleukin (IL)-2 ELISpot in addition to ESAT-6 and CFP-10 specific IFN-γ ELISpot and tuberculin skin testing (TST). Results of the TST, IFN-γ ELISpot and IL-2 ELISpot were positive in 6/172 (3.4%), 7/167 (4.2%) and 6/196 (3.1%) of contacts, respectively. Close contact (≥100 hours) to the index case increased the risk of positive results in the IFN-γ ELISpot, TST, and IL-2 ELISpot by 40.8, 19.3, and 2.5 times, respectively. Individuals with a positive IFN-γ ELISpot/negative IL-2 ELISpot result had a median (IQR) duration of index case exposure of 568 hours (133_1000) compared to individuals with a positive IFN-γ ELISpot/positive IL-2 ELISpot result (median=24 hours; 20_130; p-value=0.047). Combination of aM. tuberculosisspecific IFN-γ ELISpot with aM. tuberculosisspecific IL-2 ELISpot significantly improved the identification of individuals with the highest risk of recentM. tuberculosisinfection and is a promising method that should be explored to target tuberculosis preventive chemotherapy.File | Dimensione | Formato | |
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