Nine transformants ofTrichoderma longibrachiatumwith extra copies of theegl1gene were studied for mitotic stability, endoglucanase production, and biocontrol activity againstPythium ultimumon cucumber seedlings. The transformants showed a significantly higher level of expression of theegl1gene in comparison to the wild type under both inducing and noninducing growth conditions. Transformants with theegl1gene under the control of a constitutive promoter had the highest enzymatic activity. Both the endoglucanase activity and the transforming sequences were stable under nonselective conditions. When applied to cucumber seeds sown inP. ultimum-infested soil,T. longibrachiatumtransformants with increased inducible or constitutiveegl1expression generally were more suppressive than the wild-type strain.

Transformants ofTrichoderma longibrachiatumoverexpressing the beta-1,4-endoglucanase geneegl1show enhanced biocontrol ofPythium ultimumon cucumber / Migheli, Quirico. - In: PHYTOPATHOLOGY. - ISSN 0031-949X. - 88:7(1998), pp. 673-677. [10.1094/PHYTO.1998.88.7.673]

Transformants ofTrichoderma longibrachiatumoverexpressing the beta-1,4-endoglucanase geneegl1show enhanced biocontrol ofPythium ultimumon cucumber

Migheli, Quirico;Ramón-Vidal, Daniel
1998

Abstract

Nine transformants ofTrichoderma longibrachiatumwith extra copies of theegl1gene were studied for mitotic stability, endoglucanase production, and biocontrol activity againstPythium ultimumon cucumber seedlings. The transformants showed a significantly higher level of expression of theegl1gene in comparison to the wild type under both inducing and noninducing growth conditions. Transformants with theegl1gene under the control of a constitutive promoter had the highest enzymatic activity. Both the endoglucanase activity and the transforming sequences were stable under nonselective conditions. When applied to cucumber seeds sown inP. ultimum-infested soil,T. longibrachiatumtransformants with increased inducible or constitutiveegl1expression generally were more suppressive than the wild-type strain.
Transformants ofTrichoderma longibrachiatumoverexpressing the beta-1,4-endoglucanase geneegl1show enhanced biocontrol ofPythium ultimumon cucumber / Migheli, Quirico. - In: PHYTOPATHOLOGY. - ISSN 0031-949X. - 88:7(1998), pp. 673-677. [10.1094/PHYTO.1998.88.7.673]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/262994
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