In this study the enzymatic activity ofMycoplasma agalactiaeMAG_5040, a magnesium-dependent nuclease homologue to the staphylococcal SNase was characterized and its antigenicity during natural infections was established. A UGA corrected version of MAG_5040, lacking the region encoding the signal peptide, was expressed inEscherichia colias a GST fusion protein. Recombinant GST-MAG_5040 exhibits nuclease activity similar to typical sugar-nonspecific endo- and exonucleases, with DNA as the preferred substrate and optimal activity in the presence of 20 mM MgCl2at temperatures ranging from 37 to 45uC. According toin silicoanalyses, the position of the gene encoding MAG_5040 is consistently located upstream an ABC transporter, in most sequenced mycoplasmas belonging to theMycoplasma hominisgroup. InM. agalactiae, MAG_5040 is transcribed in a polycistronic RNA together with the ABC transporter components and with MAG_5030, which is predicted to be a sugar solute binding protein by 3D modeling and homology search. In a natural model of sheep and goats infection, anti-MAG_5040 antibodies were detected up to 9 months post infection. Taking into account its enzymatic activity, MAG_5040 could play a key role inMycoplasma agalactiaesurvival into the host, contributing to host pathogenicity. The identification of MAG_5040 opens new perspectives for the development of suitable tools for the control of contagious agalactia in small ruminants.

Mycoplasma agalactiaeMAG_5040 is a Mg2+-dependent, sugar-nonspecific SNase recognised by the host humoral response during natural infection / Uzzau, Sergio; Chessa, Bernardo; Pittau, Marco; Alberti, Alberto. - 8:2(2013). [10.1371/journal.pone.0057775]

Mycoplasma agalactiaeMAG_5040 is a Mg2+-dependent, sugar-nonspecific SNase recognised by the host humoral response during natural infection

Uzzau, Sergio;Chessa, Bernardo;Pittau, Marco;Coradduzza, Elisabetta;Nuvoli, Anna Maria;Alberti, Alberto;Addis, Maria Filippa;Tore, Gessica;Dore, Gian Mario
2013

Abstract

In this study the enzymatic activity ofMycoplasma agalactiaeMAG_5040, a magnesium-dependent nuclease homologue to the staphylococcal SNase was characterized and its antigenicity during natural infections was established. A UGA corrected version of MAG_5040, lacking the region encoding the signal peptide, was expressed inEscherichia colias a GST fusion protein. Recombinant GST-MAG_5040 exhibits nuclease activity similar to typical sugar-nonspecific endo- and exonucleases, with DNA as the preferred substrate and optimal activity in the presence of 20 mM MgCl2at temperatures ranging from 37 to 45uC. According toin silicoanalyses, the position of the gene encoding MAG_5040 is consistently located upstream an ABC transporter, in most sequenced mycoplasmas belonging to theMycoplasma hominisgroup. InM. agalactiae, MAG_5040 is transcribed in a polycistronic RNA together with the ABC transporter components and with MAG_5030, which is predicted to be a sugar solute binding protein by 3D modeling and homology search. In a natural model of sheep and goats infection, anti-MAG_5040 antibodies were detected up to 9 months post infection. Taking into account its enzymatic activity, MAG_5040 could play a key role inMycoplasma agalactiaesurvival into the host, contributing to host pathogenicity. The identification of MAG_5040 opens new perspectives for the development of suitable tools for the control of contagious agalactia in small ruminants.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/262709
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