Introduction: Typical EPEC are considered a leading cause of diarrhoea in developing countries, while atypical EPEC have been isolated more frequently in developed areas. The actual geographic distribution of the two EPEC subgroups is controversial, since data can be highly influenced by laboratory resources. This study aimed to compare the distribution of typical and atypical EPEC among children in developed and developing countries, and to characterize the bacterial isolates, using a unique methodological approach.Methodology: A total of 1,049E. coliwere isolated from faeces of children with acute diarrhoea in Mozambique, Angola and Italy, and processed by PCR to assess the presence of a large panel of virulence genes. All isolates classified as EPEC were further characterized by evaluating adherence and capability to induce actin rearrangement on Hep-2 cells.Results: Overall we isolated 59 EPEC, likewise distributed in the three countries, representing the 5.04%, 4.44% and 6.97% of all Mozambican, Angolan and Italian isolates, respectively. Nevertheless, the geographic distribution of the two EPEC subgroups was not homogeneous: in Italy we isolated 28 aEPEC but no tEPEC, while in Angola and Mozambique the percentage of the two subgroups was comparable. Twelve atypical EPEC were FAS positive and able to induce localized-like adherence on Hep-2 cells, but no correlation with the geographic origin of isolates was observed.Conclusion: Atypical EPEC are present in sub-Saharan areas in a percentage similar to that of typical strains, and are not mainly restricted to industrialized countries, as it was previously supposed.

Genotypic and phenotypic features of enteropathogenicEscherichia coliisolated in industrialized and developing countries / Fiori, Pier Luigi; Santona, Simona; Diaz, Nicia Maria Luisa Eugenia; Francisco, Moises; Sidat, Mohsin; Rappelli, Paola; Cappuccinelli, Pietro Antonio. - 7:3(2013), pp. 214-219. [10.3855/jidc.3054]

Genotypic and phenotypic features of enteropathogenicEscherichia coliisolated in industrialized and developing countries

Fiori, Pier Luigi;Santona, Simona;Rappelli, Paola;Cappuccinelli, Pietro Antonio
2013-01-01

Abstract

Introduction: Typical EPEC are considered a leading cause of diarrhoea in developing countries, while atypical EPEC have been isolated more frequently in developed areas. The actual geographic distribution of the two EPEC subgroups is controversial, since data can be highly influenced by laboratory resources. This study aimed to compare the distribution of typical and atypical EPEC among children in developed and developing countries, and to characterize the bacterial isolates, using a unique methodological approach.Methodology: A total of 1,049E. coliwere isolated from faeces of children with acute diarrhoea in Mozambique, Angola and Italy, and processed by PCR to assess the presence of a large panel of virulence genes. All isolates classified as EPEC were further characterized by evaluating adherence and capability to induce actin rearrangement on Hep-2 cells.Results: Overall we isolated 59 EPEC, likewise distributed in the three countries, representing the 5.04%, 4.44% and 6.97% of all Mozambican, Angolan and Italian isolates, respectively. Nevertheless, the geographic distribution of the two EPEC subgroups was not homogeneous: in Italy we isolated 28 aEPEC but no tEPEC, while in Angola and Mozambique the percentage of the two subgroups was comparable. Twelve atypical EPEC were FAS positive and able to induce localized-like adherence on Hep-2 cells, but no correlation with the geographic origin of isolates was observed.Conclusion: Atypical EPEC are present in sub-Saharan areas in a percentage similar to that of typical strains, and are not mainly restricted to industrialized countries, as it was previously supposed.
2013
Genotypic and phenotypic features of enteropathogenicEscherichia coliisolated in industrialized and developing countries / Fiori, Pier Luigi; Santona, Simona; Diaz, Nicia Maria Luisa Eugenia; Francisco, Moises; Sidat, Mohsin; Rappelli, Paola; Cappuccinelli, Pietro Antonio. - 7:3(2013), pp. 214-219. [10.3855/jidc.3054]
File in questo prodotto:
File Dimensione Formato  
Santona_S_Genotypic_phenotypic_features_enteropathogenic.pdf

accesso aperto

Tipologia: Versione editoriale (versione finale pubblicata)
Licenza: Creative commons
Dimensione 492.47 kB
Formato Adobe PDF
492.47 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/262241
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 14
  • ???jsp.display-item.citation.isi??? 12
social impact