A study on presence of antibodies toTrichomonis vaginalisin serum was done on a group of 500 pregnant, asymptomatic Angolan women. A serologic screening, done by ELISA, revealed that 41% of the women had IgG and IgM against the parasite. Analysis of sera by immunoblotting revealed that 94.4% of sera with anti-T. vaginalisIgG class antibodies were reactive against a common immunogenic protein of 115 kDa. The common immunogen was identified as the protozoan α-actinin. All sera recognizing the 115-kDa antigen were reactive against both native and recombinantT. vaginalisα-actinin and nonreactive against human α-actinin. The findings presented in this work offer a new tool for epidemiologic studies and open new perspectives for vaccination.

Identification ofTrichomonas vaginalisalpha-actinin as the most common immunogen recognized by sera of women exposed to the parasite / Cappuccinelli, Pietro Antonio; Fiori, Pier Luigi; Addis, Maria Filippa; Rapelli, Paola; Pinto de Andrade, Ana Maria; Madeira Rita, Fatima; Colombo, Mauro Maria. - In: THE JOURNAL OF INFECTIOUS DISEASES. - ISSN 0022-1899. - 180:5(1999), pp. 1727-1730. [10.1086/315095]

Identification ofTrichomonas vaginalisalpha-actinin as the most common immunogen recognized by sera of women exposed to the parasite

Cappuccinelli, Pietro Antonio;Fiori, Pier Luigi;Addis, Maria Filippa;Colombo, Mauro Maria
1999

Abstract

A study on presence of antibodies toTrichomonis vaginalisin serum was done on a group of 500 pregnant, asymptomatic Angolan women. A serologic screening, done by ELISA, revealed that 41% of the women had IgG and IgM against the parasite. Analysis of sera by immunoblotting revealed that 94.4% of sera with anti-T. vaginalisIgG class antibodies were reactive against a common immunogenic protein of 115 kDa. The common immunogen was identified as the protozoan α-actinin. All sera recognizing the 115-kDa antigen were reactive against both native and recombinantT. vaginalisα-actinin and nonreactive against human α-actinin. The findings presented in this work offer a new tool for epidemiologic studies and open new perspectives for vaccination.
Identification ofTrichomonas vaginalisalpha-actinin as the most common immunogen recognized by sera of women exposed to the parasite / Cappuccinelli, Pietro Antonio; Fiori, Pier Luigi; Addis, Maria Filippa; Rapelli, Paola; Pinto de Andrade, Ana Maria; Madeira Rita, Fatima; Colombo, Mauro Maria. - In: THE JOURNAL OF INFECTIOUS DISEASES. - ISSN 0022-1899. - 180:5(1999), pp. 1727-1730. [10.1086/315095]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/262076
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