A lot of evidence demonstrates that sheep could represent an experimental model to set up medical procedures in view of their application on humans. Sheep are chosen as models for human biomechanical studies because their skeleton has some similarities to humans. The aim of this work was to set up sheep primary cultures from ovine fetuses at different ages, from pregnant uteri retrieved at local abattoirs. Cell characterization showed that one cell population was immunopositive to GFAP and identifiable as astrocytes, whereas a second cell type was III β-tubulin-positive, and hence classified as neurons. At 60-day old fetus is suitable to obtain neurons, whereas in a 90-day old fetus the cell culture is predominantly characterized by glial cells. The procedure here proposed is inexpensive, in fact, collecting fetuses during sheep slaughtering is a costsaving option, unlike common experimental animals such as mice, rats, rabbits, that require very high economical efforts. Finally, our protocol fully eliminates the need of animal killing, being living animals replaced by a validated in vitro model in agreement with the 3Rs statement.

Ovine fetuses from slaughterhouses: A useful source for neural cell primary cultures / Farina, Vittorio; Gadau, Sergio Domenico; Lepore, Gianluca; Carcupino, Marcella; Zedda, Marco. - In: JOURNAL OF BIOLOGICAL RESEARCH. - ISSN 2284-0230. - (2021). [10.4081/jbr.2021.9344]

Ovine fetuses from slaughterhouses: A useful source for neural cell primary cultures

Farina, Vittorio;Gadau, Sergio Domenico;Lepore, Gianluca;Carcupino, Marcella;Zedda, Marco
2021

Abstract

A lot of evidence demonstrates that sheep could represent an experimental model to set up medical procedures in view of their application on humans. Sheep are chosen as models for human biomechanical studies because their skeleton has some similarities to humans. The aim of this work was to set up sheep primary cultures from ovine fetuses at different ages, from pregnant uteri retrieved at local abattoirs. Cell characterization showed that one cell population was immunopositive to GFAP and identifiable as astrocytes, whereas a second cell type was III β-tubulin-positive, and hence classified as neurons. At 60-day old fetus is suitable to obtain neurons, whereas in a 90-day old fetus the cell culture is predominantly characterized by glial cells. The procedure here proposed is inexpensive, in fact, collecting fetuses during sheep slaughtering is a costsaving option, unlike common experimental animals such as mice, rats, rabbits, that require very high economical efforts. Finally, our protocol fully eliminates the need of animal killing, being living animals replaced by a validated in vitro model in agreement with the 3Rs statement.
Ovine fetuses from slaughterhouses: A useful source for neural cell primary cultures / Farina, Vittorio; Gadau, Sergio Domenico; Lepore, Gianluca; Carcupino, Marcella; Zedda, Marco. - In: JOURNAL OF BIOLOGICAL RESEARCH. - ISSN 2284-0230. - (2021). [10.4081/jbr.2021.9344]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/246934
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
social impact