Background: The association between head and neck squamous cell carcinoma (HNSCC) and allelic variants of glutathione S-transferase M1 (GSTM1) and -T1 (GSTT1) is currently controversial. The present study investigates the prevalences of GSTT1 and GSTM1 polymorphism in a cohort of 100 head and neck cancer patients, 100 healthy donors and 200 controls with non-neoplastic head and neck diseases from Italian Lazio Region. Methods: The patients with benign head and neck pathologies, as well as the healthy donors were matched for age, sex, cigarette smoke (yes/no) and alcohol consumption (yes/no). Molecular definition of GSTT1 and GSTM1 genotype has been performed by means of allele-specific PCR technique. Results: A significant association between head and neck cancer and GSTM1 null genotype was observed both considering benign disease controls (p = 0.001, OR = 2.613; 95% C.I. = 1.48-4.62), and healthy donors (p = 0.0003, OR = 3.35; 95% C.I. 1.69-6.67) while no significant association was found with GSTT1 null genotype (p ≥ 0.14). No interactive association was observed when combining the different genotypes of the two polymorphisms. These results were confirmed after correction for daily number of cigarettes and period of tobacco exposure. Conclusions: The present study confirms a role for genetic alterations of GSTM1 detoxifying enzyme as a risk factor for the development of HNSCC in patients from the Italian Lazio Region, independently of age, sex and other confounding variables. © 2006 Elsevier B.V. All rights reserved.
GSTT1 and GSTM1 allelic polymorphisms in head and neck cancer patients from Italian Lazio Region / Capoluongo, E.; Almadori, G.; Concolino, P.; Bussu, F.; Santonocito, C.; Vendittelli, F.; Galli, J.; Zuppi, C.; Ameglio, F.; Paludetti, G.; Giardina, B.. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - 376:1-2(2007), pp. 174-178. [10.1016/j.cca.2006.08.017]
GSTT1 and GSTM1 allelic polymorphisms in head and neck cancer patients from Italian Lazio Region
Bussu F.;
2007-01-01
Abstract
Background: The association between head and neck squamous cell carcinoma (HNSCC) and allelic variants of glutathione S-transferase M1 (GSTM1) and -T1 (GSTT1) is currently controversial. The present study investigates the prevalences of GSTT1 and GSTM1 polymorphism in a cohort of 100 head and neck cancer patients, 100 healthy donors and 200 controls with non-neoplastic head and neck diseases from Italian Lazio Region. Methods: The patients with benign head and neck pathologies, as well as the healthy donors were matched for age, sex, cigarette smoke (yes/no) and alcohol consumption (yes/no). Molecular definition of GSTT1 and GSTM1 genotype has been performed by means of allele-specific PCR technique. Results: A significant association between head and neck cancer and GSTM1 null genotype was observed both considering benign disease controls (p = 0.001, OR = 2.613; 95% C.I. = 1.48-4.62), and healthy donors (p = 0.0003, OR = 3.35; 95% C.I. 1.69-6.67) while no significant association was found with GSTT1 null genotype (p ≥ 0.14). No interactive association was observed when combining the different genotypes of the two polymorphisms. These results were confirmed after correction for daily number of cigarettes and period of tobacco exposure. Conclusions: The present study confirms a role for genetic alterations of GSTM1 detoxifying enzyme as a risk factor for the development of HNSCC in patients from the Italian Lazio Region, independently of age, sex and other confounding variables. © 2006 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
