The inhibitory action that a Brevibacillus laterosporus strain isolated from the honeybee body causes against the American Foulbrood (AFB) etiological agent Paenibacillus larvae was studied by in-vitro experiments. A protein fraction isolated from B. laterosporus culture supernatant was involved in the observed inhibition of P. larvae vegetative growth and spore germination. As a result of LC-MS/MS proteomic analyses, the bacteriocin laterosporulin was found to be the major component of this fraction, followed by other antimicrobial proteins and substances including lectins, chaperonins, various enzymes and a number of putative uncharacterized proteins. The results obtained in this study highlight the potential of B. laterosporus as a biological control agent for preserving and improving honeybee health.
Inhibition of Paenibacillus larvae by an extracellular protein fraction from a honeybee-borne Brevibacillus laterosporus strain / Marche, M. G.; Satta, A.; Floris, I.; Lazzeri, A. M.; Ruiu, L.. - In: MICROBIOLOGICAL RESEARCH. - ISSN 0944-5013. - 227:(2019), p. 126303. [10.1016/j.micres.2019.126303]