Mycoplasma lipoproteins play a relevant role in pathogenicity and directly interact with the host immune system. Among human mycoplasmas, Mycoplasma hominis is described as a commensal bacterium that can be associated with a number of genital and extragenital conditions. Mechanisms of M. hominis pathogenicity are still largely obscure, and only a limited number of proteins have been associated with virulence. The current study focused on investigating the role of MHO_0730 as a virulence factor and demonstrated that MHO_0730 is a surface lipoprotein, potentially expressed in vivo during natural infection, acting both as a nuclease with its amino acidic portion and as a potent inducer of Neutrophil extracellular trapsosis with its N-terminal lipid moiety. Evidence for M. hominis neutrophil extracellular trap escape is also presented. Results highlight the relevance of MHO_0730 in promoting infection and modulation and evasion of innate immunity and provide additional knowledge on M. hominis virulence and survival in the host.

MHO_0730 as a Surface-Exposed Calcium-Dependent Nuclease of Mycoplasma hominis Promoting Neutrophil Extracellular Trap Formation and Escape / Cacciotto, Carla; Dessi', Daniele; Cubeddu, Tiziana; Cocco, Anna Rita; Pisano, Andrea; Tore, Gessica; Fiori, Pier Luigi; Rappelli, Paola Giovanna Francesca; Pittau, Marco; Alberti, Alberto. - In: THE JOURNAL OF INFECTIOUS DISEASES. - ISSN 1537-6613. - 220:12(2019), pp. 1999-2008. [10.1093/infdis/jiz406]

MHO_0730 as a Surface-Exposed Calcium-Dependent Nuclease of Mycoplasma hominis Promoting Neutrophil Extracellular Trap Formation and Escape

Carla Cacciotto;Daniele Dessì;Tiziana Cubeddu;Anna Rita Cocco;Andrea Pisano;Gessica Tore;Pier Luigi Fiori;Paola Rappelli;Marco Pittau;Alberto Alberti
2019

Abstract

Mycoplasma lipoproteins play a relevant role in pathogenicity and directly interact with the host immune system. Among human mycoplasmas, Mycoplasma hominis is described as a commensal bacterium that can be associated with a number of genital and extragenital conditions. Mechanisms of M. hominis pathogenicity are still largely obscure, and only a limited number of proteins have been associated with virulence. The current study focused on investigating the role of MHO_0730 as a virulence factor and demonstrated that MHO_0730 is a surface lipoprotein, potentially expressed in vivo during natural infection, acting both as a nuclease with its amino acidic portion and as a potent inducer of Neutrophil extracellular trapsosis with its N-terminal lipid moiety. Evidence for M. hominis neutrophil extracellular trap escape is also presented. Results highlight the relevance of MHO_0730 in promoting infection and modulation and evasion of innate immunity and provide additional knowledge on M. hominis virulence and survival in the host.
MHO_0730 as a Surface-Exposed Calcium-Dependent Nuclease of Mycoplasma hominis Promoting Neutrophil Extracellular Trap Formation and Escape / Cacciotto, Carla; Dessi', Daniele; Cubeddu, Tiziana; Cocco, Anna Rita; Pisano, Andrea; Tore, Gessica; Fiori, Pier Luigi; Rappelli, Paola Giovanna Francesca; Pittau, Marco; Alberti, Alberto. - In: THE JOURNAL OF INFECTIOUS DISEASES. - ISSN 1537-6613. - 220:12(2019), pp. 1999-2008. [10.1093/infdis/jiz406]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/228533
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