Nutraceuticals present in food are molecules able to exert biological activity for the prevention and treatment of various diseases, in form of pharmaceutical preparations, such as capsules, cream, or pills. Myrtus communis L. is a spontaneous Mediterranean evergreen shrub, widely known for the liqueur obtained from its berries rich in phytochemicals such as tannins and flavonoids. In the present study, we aimed to evaluate the properties of myrtle byproducts, residual of the industrial liqueur processing, in Adipose-derived stem cells (ADSCs) induced at oxidative stress by in vitro H2O2 treatment. Cells were exposed for 12-24 and 48h at treatment with extracts and then senescence-induced. ROS production was then determined.The real-time PCR was performed to evaluate the expression of inflammatory cytokines and sirtuin-dependent epigenetic changes, as well the modifications in terms of stem cell pluripotency. The -galactosidase assay was conducted to analyze stem cell senescence after treatment. Our results show that industrial myrtle byproducts retain a high antioxidant and antisenescence activity, protecting cells from oxidative stress damages. The results obtained suggest that residues from myrtle liqueur production could be used as resource in formulation of food supplements or pharmaceutical preparations with antioxidant, antiaging, and anti-inflammatory activity.

Extracts from myrtle liqueur processing waste modulate stem cells pluripotency under stress conditions / Cruciani, Sara; Santaniello, Sara; Fadda, Angela; Sale, Luana; Sarais, Giorgia; Sanna, Daniele; Mulas, Maurizio; Ginesu, Giorgio Carlo; Cossu, Maria Laura; Serra, Pier Andrea; Ventura, Carlo; Maioli, Margherita. - In: BIOMED RESEARCH INTERNATIONAL. - ISSN 2314-6141. - 2019:(2019), pp. 1-12. [10.1155/2019/5641034]

Extracts from myrtle liqueur processing waste modulate stem cells pluripotency under stress conditions

Cruciani, Sara
Membro del Collaboration Group
;
Santaniello, Sara
Membro del Collaboration Group
;
Mulas, Maurizio
Membro del Collaboration Group
;
Ginesu, Giorgio Carlo
Membro del Collaboration Group
;
Cossu, Maria Laura
Membro del Collaboration Group
;
Serra, Pier Andrea
Membro del Collaboration Group
;
Maioli, Margherita
Membro del Collaboration Group
2019

Abstract

Nutraceuticals present in food are molecules able to exert biological activity for the prevention and treatment of various diseases, in form of pharmaceutical preparations, such as capsules, cream, or pills. Myrtus communis L. is a spontaneous Mediterranean evergreen shrub, widely known for the liqueur obtained from its berries rich in phytochemicals such as tannins and flavonoids. In the present study, we aimed to evaluate the properties of myrtle byproducts, residual of the industrial liqueur processing, in Adipose-derived stem cells (ADSCs) induced at oxidative stress by in vitro H2O2 treatment. Cells were exposed for 12-24 and 48h at treatment with extracts and then senescence-induced. ROS production was then determined.The real-time PCR was performed to evaluate the expression of inflammatory cytokines and sirtuin-dependent epigenetic changes, as well the modifications in terms of stem cell pluripotency. The -galactosidase assay was conducted to analyze stem cell senescence after treatment. Our results show that industrial myrtle byproducts retain a high antioxidant and antisenescence activity, protecting cells from oxidative stress damages. The results obtained suggest that residues from myrtle liqueur production could be used as resource in formulation of food supplements or pharmaceutical preparations with antioxidant, antiaging, and anti-inflammatory activity.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11388/222773
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