Currently, several commercially available biochemical kits are validated for their use in human but not in animals. The purpose of this work is to demonstrate the applicability of human kits for alanine-aminotransferase, aspartato-aminotransferase, albumin, total protein, total cholesterol, and triglycerides in ovine plasma. Assays were validated according to international guidelines and stability was explored. Accuracy values were between 67 and 100%, and intra and interday precisions (%RSD) were <15% for all studied parameters. These results confirm the suitability of the studied human kits for their use in ovine plasma and they were used in plasma collected from pregnant ewes.
Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma / Pasciu, Valeria; Baralla, Elena; Nieddu, Maria; Succu, Sara; Porcu, Cristian; Leoni, Giovanni G.; Sechi, Pietro; Bomboi, Giovanni C.; Berlinguer, Fiammetta. - In: JOURNAL OF VETERINARY MEDICAL SCIENCE. - ISSN 0916-7250. - 81:2(2019), pp. 294-297. [10.1292/jvms.18-0356]
Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma
Pasciu, Valeria;Baralla, Elena;Nieddu, Maria;Succu, Sara;Porcu, Cristian;Leoni, Giovanni G.;Bomboi, Giovanni C.;Berlinguer, Fiammetta
2019-01-01
Abstract
Currently, several commercially available biochemical kits are validated for their use in human but not in animals. The purpose of this work is to demonstrate the applicability of human kits for alanine-aminotransferase, aspartato-aminotransferase, albumin, total protein, total cholesterol, and triglycerides in ovine plasma. Assays were validated according to international guidelines and stability was explored. Accuracy values were between 67 and 100%, and intra and interday precisions (%RSD) were <15% for all studied parameters. These results confirm the suitability of the studied human kits for their use in ovine plasma and they were used in plasma collected from pregnant ewes.File | Dimensione | Formato | |
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pasciu - commercial human kits applicabiliby for the determination fo biochemical parameters in sheep plasma.pdf
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