Currently, several commercially available biochemical kits are validated for their use in human but not in animals. The purpose of this work is to demonstrate the applicability of human kits for alanine-aminotransferase, aspartato-aminotransferase, albumin, total protein, total cholesterol, and triglycerides in ovine plasma. Assays were validated according to international guidelines and stability was explored. Accuracy values were between 67 and 100%, and intra and interday precisions (%RSD) were <15% for all studied parameters. These results confirm the suitability of the studied human kits for their use in ovine plasma and they were used in plasma collected from pregnant ewes.

Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma / Pasciu, Valeria; Baralla, Elena; Nieddu, Maria; Succu, Sara; Porcu, Cristian; Leoni, Giovanni G.; Sechi, Pietro; Bomboi, Giovanni C.; Berlinguer, Fiammetta. - In: JOURNAL OF VETERINARY MEDICAL SCIENCE. - ISSN 0916-7250. - 81:2(2019), pp. 294-297. [10.1292/jvms.18-0356]

Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma

Pasciu, Valeria;Baralla, Elena;Nieddu, Maria;Succu, Sara;Porcu, Cristian;Leoni, Giovanni G.;Bomboi, Giovanni C.;Berlinguer, Fiammetta
2019-01-01

Abstract

Currently, several commercially available biochemical kits are validated for their use in human but not in animals. The purpose of this work is to demonstrate the applicability of human kits for alanine-aminotransferase, aspartato-aminotransferase, albumin, total protein, total cholesterol, and triglycerides in ovine plasma. Assays were validated according to international guidelines and stability was explored. Accuracy values were between 67 and 100%, and intra and interday precisions (%RSD) were <15% for all studied parameters. These results confirm the suitability of the studied human kits for their use in ovine plasma and they were used in plasma collected from pregnant ewes.
2019
Commercial human kits’ applicability for the determination of biochemical parameters in sheep plasma / Pasciu, Valeria; Baralla, Elena; Nieddu, Maria; Succu, Sara; Porcu, Cristian; Leoni, Giovanni G.; Sechi, Pietro; Bomboi, Giovanni C.; Berlinguer, Fiammetta. - In: JOURNAL OF VETERINARY MEDICAL SCIENCE. - ISSN 0916-7250. - 81:2(2019), pp. 294-297. [10.1292/jvms.18-0356]
File in questo prodotto:
File Dimensione Formato  
pasciu - commercial human kits applicabiliby for the determination fo biochemical parameters in sheep plasma.pdf

accesso aperto

Tipologia: Versione editoriale (versione finale pubblicata)
Licenza: Creative commons
Dimensione 1.09 MB
Formato Adobe PDF
1.09 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11388/220997
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 4
social impact