Objective To study the molecular features of mesenchymal stem cells from Wharton Jelly (WJ-MSCs) of umbilical cord to predict their differentiation capacity. Design Comparison of gene expression from mesenchymal stem cells of male and female umbilical cord Setting University hospital Patient (s) umbilical cords (n=12, 6 males and 6 females) retrieved from spontaneous full-term vaginal delivery of healthy women Intervention we analyzed the expression of the stemness related genes C-MYC, OCT4, SOX2 and NANOG and of the epigenetic modulating gene DNA-methyltransferase 1 (DNMT1). Mean outcome measure WJ-MSCs were isolated by standard procedures and immunophenotypically characterized. Gene expression analysis of stemness related genes and the epigenetic modulating gene DNMT1 were performed by real-time PCR Results expression of the OCT4 and DNMT1 genes was significantly higher in WJ- MSCs isolated from male subjects, as compared to MSCs isolated from female-derived WJ. The resulting higher expression of OCT4 and DNMT1 in WJ-MSCs from males as compared with female WJ-MSCs for the first time identifies a specific relationship between stemness genes, an epigenetic modulator, and gender differences. Conclusion our findings disclose novel biomedical implications in WJ-MSCs related to the sex of the donor, thus providing additional cues to exploit their regenerative potential in allogenic transplantation.
Lessons from human umbilical cord: gender differences in stem cells from Wharton’s jelly / Balzano, Francesca; Bellu, Emanuela; Basoli, Valentina; Giudici, Silvia Dei; Santaniello, Sara; Cruciani, Sara; Facchin, Federica; Oggiano, Annalisa; Capobianco, Giampiero; Dessole, Francesco; Ventura, Carlo; Dessole, Salvatore; Maioli, Margherita. - In: EUROPEAN JOURNAL OF OBSTETRICS, GYNECOLOGY, AND REPRODUCTIVE BIOLOGY. - ISSN 0301-2115. - 234(2019), pp. 143-148. [10.1016/j.ejogrb.2018.12.028]
Lessons from human umbilical cord: gender differences in stem cells from Wharton’s jelly
Cruciani, SaraFormal Analysis
;Capobianco, Giampiero
Writing – Review & Editing
;Dessole, SalvatoreSupervision
;Maioli, MargheritaWriting – Original Draft Preparation
2019-01-01
Abstract
Objective To study the molecular features of mesenchymal stem cells from Wharton Jelly (WJ-MSCs) of umbilical cord to predict their differentiation capacity. Design Comparison of gene expression from mesenchymal stem cells of male and female umbilical cord Setting University hospital Patient (s) umbilical cords (n=12, 6 males and 6 females) retrieved from spontaneous full-term vaginal delivery of healthy women Intervention we analyzed the expression of the stemness related genes C-MYC, OCT4, SOX2 and NANOG and of the epigenetic modulating gene DNA-methyltransferase 1 (DNMT1). Mean outcome measure WJ-MSCs were isolated by standard procedures and immunophenotypically characterized. Gene expression analysis of stemness related genes and the epigenetic modulating gene DNMT1 were performed by real-time PCR Results expression of the OCT4 and DNMT1 genes was significantly higher in WJ- MSCs isolated from male subjects, as compared to MSCs isolated from female-derived WJ. The resulting higher expression of OCT4 and DNMT1 in WJ-MSCs from males as compared with female WJ-MSCs for the first time identifies a specific relationship between stemness genes, an epigenetic modulator, and gender differences. Conclusion our findings disclose novel biomedical implications in WJ-MSCs related to the sex of the donor, thus providing additional cues to exploit their regenerative potential in allogenic transplantation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
